Protein Info for TX73_023940 in Rhodopseudomonas palustris CGA009

Annotation: Fe-S cluster assembly protein NifU

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 328 TIGR02000: Fe-S cluster assembly protein NifU" amino acids 6 to 327 (322 residues), 286.7 bits, see alignment E=1.1e-89 PF01592: NifU_N" amino acids 9 to 125 (117 residues), 113.3 bits, see alignment E=1.4e-36 PF04324: Fer2_BFD" amino acids 134 to 183 (50 residues), 51.8 bits, see alignment 1.3e-17 PF01106: NifU" amino acids 261 to 325 (65 residues), 76 bits, see alignment E=3.2e-25

Best Hits

KEGG orthology group: K13819, NifU-like protein (inferred from 99% identity to rpt:Rpal_5090)

Predicted SEED Role

"Iron-sulfur cluster assembly scaffold protein NifU" in subsystem Nitrogen fixation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (328 amino acids)

>TX73_023940 Fe-S cluster assembly protein NifU (Rhodopseudomonas palustris CGA009)
MLGLGKFDHHFSTSANAGPLPQANAVGRFGSIGWGDAVKLMLKVEPDDGRIAQARFQAFG
CSSAIAASAALTDMITGKTIDEASGISAAAIADYLGDLPRERMYCAVMTFEALQQAIGSF
RGKAAVAEADAPPVCKCLGVGQMMIERTIRFNRLTSLDDVTAYTKAAGSCSTCFKQIESL
LARVNAEMVEDGLIPAEDAYRIGSAMPRATELKPHGAPAPAANIFAAKATPAHLRPIAKA
APRPATPPAGLVDAPPQEALIAEAVEELRPHLQRDGGDCEFVSLDGNIVYVRLSGNCVGC
QLSSVTLSGVQAKLVEKFGRPLRVVPVS