Protein Info for TX73_022105 in Rhodopseudomonas palustris CGA009

Annotation: long-chain fatty acid--CoA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00501: AMP-binding
  • amino acids 29 to 421 (393 residues), 290.5 bits, E=1.9e-90
• PF13193: AMP-binding_C
  • amino acids 472 to 546 (75 residues), 79.6 bits, E=2.7e-26

Best Hits

KEGG orthology group: K01897, long-chain acyl-CoA synthetase [EC: 6.2.1.3] (inferred from 100% identity to rpa:RPA4267)

Predicted SEED Role

"Acetoacetyl-CoA synthetase [leucine] (EC 6.2.1.16)" in subsystem HMG CoA Synthesis or Leucine Degradation and HMG-CoA Metabolism (EC 6.2.1.16)

MetaCyc Pathways

• superpathway of fatty acids biosynthesis (E. coli) (49/53 steps found)
• palmitate biosynthesis III (28/29 steps found)
• superpathway of fatty acid biosynthesis II (plant) (38/43 steps found)
• palmitate biosynthesis II (type II fatty acid synthase) (29/31 steps found)
• oleate β-oxidation (28/35 steps found)
• fatty acid salvage (6/6 steps found)
• stearate biosynthesis II (bacteria and plants) (5/6 steps found)
• long-chain fatty acid activation (1/1 steps found)
• phytol degradation (3/4 steps found)
• stearate biosynthesis IV (4/6 steps found)
• γ-linolenate biosynthesis II (animals) (1/2 steps found)
• linoleate biosynthesis II (animals) (1/2 steps found)
• alkane biosynthesis II (1/3 steps found)
• oleate biosynthesis I (plants) (1/3 steps found)
• 3-methyl-branched fatty acid α-oxidation (3/6 steps found)
• 6-gingerol analog biosynthesis (engineered) (3/6 steps found)
• octane oxidation (2/5 steps found)
• long chain fatty acid ester synthesis (engineered) (1/4 steps found)
• phosphatidylcholine acyl editing (1/4 steps found)
• wax esters biosynthesis II (1/4 steps found)
• ceramide degradation by α-oxidation (2/7 steps found)
• sphingosine and sphingosine-1-phosphate metabolism (4/10 steps found)
• stearate biosynthesis I (animals) (1/6 steps found)
• 2-deoxy-D-ribose degradation II (2/8 steps found)
• arachidonate biosynthesis III (6-desaturase, mammals) (1/7 steps found)
• capsaicin biosynthesis (1/7 steps found)
• icosapentaenoate biosynthesis II (6-desaturase, mammals) (1/7 steps found)
• icosapentaenoate biosynthesis III (8-desaturase, mammals) (1/7 steps found)
• ceramide and sphingolipid recycling and degradation (yeast) (4/16 steps found)
• sporopollenin precursors biosynthesis (4/18 steps found)
• cutin biosynthesis (1/16 steps found)
• suberin monomers biosynthesis (3/20 steps found)

KEGG Metabolic Maps

• Butanoate metabolism
• Fatty acid metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.2.1.16, 6.2.1.3

Use Curated BLAST to search for 6.2.1.16 or 6.2.1.3

Protein Sequence (563 amino acids)

>TX73_022105 long-chain fatty acid--CoA ligase (Rhodopseudomonas palustris CGA009)
MERIWLKQYPPGVPADIDVTQYPSLVDLFEESFRKYGDRKAFICMDKAITYRELDEMSVA
LGAYLQGKGLQKGARVALMMPNVLQYPVAIAAVLRAGFAVVNVNPLYTPRELEHQLKDSG
AEAIIVLENFAHTVEQVIARTPVKHIIVGAMGDMLGFKGVIVNLVVRKVKKMVPAFSLPG
AVSFNDAVSAGRGQKFTKPAIGPESVAFLQYTGGTTGVSKGATLLHRNVVANVLQNDAWL
QPALAKPPHVDQLFIVCALPLYHIFALTACYLLGMRAGGVNLLIPNPRDIPGFIKELMKY
QVNSFPAVNTLYNGLLHAPGFDKVDFSKLKISNGGGMAVQRPVAEAWLKMTGCPIAEGYG
LSETAPVLTCNPATVNEFNGTIGLPMPSTELSIRDEEGKEVPFGTAGEICAKGPQVMSGY
WNRPDETALVMTADGFFRTGDIGVMSPDGFTKIVDRKKDMILVSGFNVYPNEVEEVVASH
PGVLECAVIGVPDSRTGEAVKAFVVKKDPDVTAEDIIKFCHHELTNYKVPRQIEFRTTLP
KTNVGKILRRELRDEKKSNQAAA