Protein Info for TX73_021595 in Rhodopseudomonas palustris CGA009

Annotation: carbon-nitrogen hydrolase family protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00795: CN_hydrolase
  • amino acids 5 to 273 (269 residues), 178.9 bits, E=6e-57

Best Hits

Swiss-Prot: 53% identical to NITR2_BRADU: Nitrilase blr3397 (blr3397) from Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)

KEGG orthology group: K01501, nitrilase [EC: 3.5.5.1] (inferred from 100% identity to rpa:RPA4166)

Predicted SEED Role

"Plant-induced nitrilase (EC 3.5.5.1), hydrolyses beta-cyano-L-alanine" (EC 3.5.5.1)

MetaCyc Pathways

• indole-3-acetate biosynthesis V (bacteria and fungi) (1/1 steps found)
• indole glucosinolate activation (herbivore attack) (8/13 steps found)
• indole-3-acetate biosynthesis II (5/12 steps found)

KEGG Metabolic Maps

• Benzoate degradation via CoA ligation
• Cyanoamino acid metabolism
• Nitrogen metabolism
• Styrene degradation
• Tryptophan metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.5.5.1

Protein Sequence (317 amino acids)

>TX73_021595 carbon-nitrogen hydrolase family protein (Rhodopseudomonas palustris CGA009)
MAKLKVAAVQATTVPFDAAAATVRTVALIGEAAAKGAKIAVFPEAFIGGYPKGLDFGCAI
GRRTPEGREDFARYVRGAIAVPGPEVDQLVAACAEHDLHATVGVIERDGGTLYCTALYLA
PTGLLGIHRKIMPTGSERLVWGFGDGSTLTVVDTPYGKLGGAICWEHYMPLMRAAYYAKG
VQIWAAPTADDRESWIATMRHIALEGRCFVIGACQVMRRSDFPADYASRIEAGPDEWMMH
GRSVIVGPLGEILAGPLLDEEGILTADIDTDDILGSKLDFDAVGHYSRPDLFTLQVDERP
QTPVAFSAKTGAGDAGA