Protein Info for TX73_020645 in Rhodopseudomonas palustris CGA009

Annotation: D-glycero-beta-D-manno-heptose-7-phosphate kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 490 TIGR02198: bifunctional protein RfaE, domain I" amino acids 9 to 322 (314 residues), 378.1 bits, see alignment E=4.7e-117 PF00294: PfkB" amino acids 15 to 315 (301 residues), 142.1 bits, see alignment E=3.8e-45 PF08543: Phos_pyr_kin" amino acids 195 to 307 (113 residues), 36.4 bits, see alignment E=5.7e-13 TIGR02199: bifunctional protein RfaE, domain II" amino acids 347 to 486 (140 residues), 202.4 bits, see alignment E=4.2e-64 TIGR00125: cytidyltransferase-like domain" amino acids 356 to 421 (66 residues), 50.9 bits, see alignment E=2e-17 PF01467: CTP_transf_like" amino acids 358 to 452 (95 residues), 55.7 bits, see alignment E=9.1e-19

Best Hits

Swiss-Prot: 100% identical to HLDE_RHOPA: Bifunctional protein HldE (hldE) from Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009)

KEGG orthology group: K03272, D-beta-D-heptose 7-phosphate kinase / D-beta-D-heptose 1-phosphate adenosyltransferase [EC: 2.7.1.- 2.7.7.-] (inferred from 100% identity to rpa:RPA3984)

Predicted SEED Role

"ADP-heptose synthase (EC 2.7.-.-) / D-glycero-beta-D-manno-heptose 7-phosphate kinase" in subsystem LOS core oligosaccharide biosynthesis (EC 2.7.-.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.-.-, 2.7.1.-, 2.7.7.-

Use Curated BLAST to search for 2.7.-.- or 2.7.1.- or 2.7.7.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (490 amino acids)

>TX73_020645 D-glycero-beta-D-manno-heptose-7-phosphate kinase (Rhodopseudomonas palustris CGA009)
MFSFDALLQAIARQTVLCVGDLMLDEFVYGEVSRISPEAPAPVIAVQRSETNIGGAGNVA
RNIAAIGARCIFVGLIGDDATGRFLESELGSESRIEPVLVCDGSRPTTRKVRFVSEHFST
HMLRADWETASPAAADIEQRLLDAILPQLARADIVLLSDYAKGVLTARVIRDTIDAAKKL
GKRVIVDPKSANFAIYRGATLLTPNRKEFTAATRSAAATDDEIAAAAQDAMALAECEAML
VTKSEHGMTLVPRGGEPIHVPALPVKVRDVSGAGDTVAAVLAVVLASGANWATAMRAASA
AAAVAVSKNGTAVVTPAELRRRILPHASLAAEEKIIGSDEELDERLKQWRREGLRVGFTN
GCFDILHPGHVKVLTAARGACDRLIVGLNSDASVRRLKGESRPVQHERARAEVLAALEAV
DLVAIFEEDTPLRLITRIEPSVLVKGGDYTREQVVGHEIVAAKGGDVLLVDVLPGFSTTS
LVARAREGQS