Protein Info for TX73_020240 in Rhodopseudomonas palustris CGA009

Annotation: efflux RND transporter periplasmic adaptor subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 394 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details PF00529: CusB_dom_1" amino acids 39 to 362 (324 residues), 28.6 bits, see alignment E=2.2e-10 TIGR01730: efflux transporter, RND family, MFP subunit" amino acids 41 to 375 (335 residues), 271.5 bits, see alignment E=4.3e-85 PF16576: HlyD_D23" amino acids 64 to 294 (231 residues), 49.6 bits, see alignment E=6.4e-17 PF13533: Biotin_lipoyl_2" amino acids 68 to 114 (47 residues), 33.4 bits, see alignment 6e-12

Best Hits

Swiss-Prot: 44% identical to TTGD_PSEPT: Toluene efflux pump periplasmic linker protein TtgD (ttgD) from Pseudomonas putida (strain DOT-T1E)

KEGG orthology group: K03585, membrane fusion protein (inferred from 99% identity to rpt:Rpal_4427)

Predicted SEED Role

"RND efflux system, membrane fusion protein CmeA" in subsystem Multidrug Resistance Efflux Pumps or Multidrug efflux pump in Campylobacter jejuni (CmeABC operon)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (394 amino acids)

>TX73_020240 efflux RND transporter periplasmic adaptor subunit (Rhodopseudomonas palustris CGA009)
MTTKTKAALRAACAFGVVVSLAGCGDGKQAGADKQAPPVVVSVVTVVPETLRIVSDLPGR
IAPTRIAEVRPRVSGIIIERVFQQGTIVKQGDVLYRLDPAPFQVRVASAKATLQRAEATR
TLAAQQAERQTRLKDRDVTTVQLYDKAVAELAQAEADEASARAGLQAAELDLQYTEVRAP
ITGRIGRALITEGALFTVGGSESLATIQQLDPVYADFTQSANELLALKRAAQQSAKAGDA
IEAEVRLRFDDGASYAHRGRLLFSEASVDATTGQVTLRGEFPNPDGDLLPGMYVRVQLVQ
GEQANALAVPERALRRDPGGQAQLYVVSADRKAELRPVTPSRLVDGRWIIASGLHPGDAV
VVQGFQALSPGATVDPKPWPNAAVGATSATSQAK