Protein Info for TX73_019415 in Rhodopseudomonas palustris CGA009
Annotation: ethanolamine ammonia-lyase subunit EutB
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 50% identical to EUTB_ECOLI: Ethanolamine ammonia-lyase heavy chain (eutB) from Escherichia coli (strain K12)
KEGG orthology group: K03735, ethanolamine ammonia-lyase large subunit [EC: 4.3.1.7] (inferred from 99% identity to rpt:Rpal_4270)MetaCyc: 50% identical to ethanolamine ammonia-lyase subunit alpha (Escherichia coli K-12 substr. MG1655)
Ethanolamine ammonia-lyase. [EC: 4.3.1.7]
Predicted SEED Role
"Ethanolamine ammonia-lyase heavy chain (EC 4.3.1.7)" in subsystem Ethanolamine utilization (EC 4.3.1.7)
MetaCyc Pathways
- ethanolamine utilization (4/5 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 4.3.1.7
Use Curated BLAST to search for 4.3.1.7
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (460 amino acids)
>TX73_019415 ethanolamine ammonia-lyase subunit EutB (Rhodopseudomonas palustris CGA009) MIYRHSIGNVSFVFDDLRDLLAKASPPRSGDRLAGIAADSAEQMVAARMALANLPLRQFL SEMVIPYEADEVTRLIADRHDAAAFAPVASLTVGGFRDWLLSDAATPVALAALAPGLTPE MVAAVSKLMRNQDLILVAKKCSVVTRFRNTIGLPGRMSVRLQPNHPFDDARGITASILDG LLLGAGDACIGINPASDDPAVLGQLVRLLDDIIAHLAIPTQGCVLTHVTTTLRLIEQGAP VDLTFQSIAGTEAANRSFGIDLALLAEAHQATLAQKRGTVGDNVMYFETGQGSALSANAH HGVDQQTCEARAYAVARAFSPLLVNSVVGFIGPEYLYDGKQIVRAGLEDHFCGKLLGLPL GVDICYTNHAEADQDDMDTLLTLLAAAGVNFIMGVPGADDVMLNYQSTSFHDALYVRDLF GLKRAPEFDDWLIRAGFSGPDHRPLTDGALLPEIAARLIA