Protein Info for TX73_018320 in Rhodopseudomonas palustris CGA009

Annotation: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2, 6-diaminopimelate ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 485 TIGR01085: UDP-N-acetylmuramyl-tripeptide synthetase" amino acids 20 to 480 (461 residues), 440.3 bits, see alignment E=4.9e-136 PF01225: Mur_ligase" amino acids 22 to 95 (74 residues), 39.1 bits, see alignment E=1.2e-13 PF08245: Mur_ligase_M" amino acids 105 to 310 (206 residues), 155.9 bits, see alignment E=2e-49 PF02875: Mur_ligase_C" amino acids 332 to 414 (83 residues), 89.6 bits, see alignment E=2e-29

Best Hits

Swiss-Prot: 76% identical to MURE_BRADU: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase (murE) from Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)

KEGG orthology group: K01928, UDP-N-acetylmuramoylalanyl-D-glutamate--2,6-diaminopimelate ligase [EC: 6.3.2.13] (inferred from 100% identity to rpa:RPA3535)

Predicted SEED Role

"UDP-N-acetylmuramoylalanyl-D-glutamate--2,6-diaminopimelate ligase (EC 6.3.2.13)" in subsystem Methicillin resistance in Staphylococci or Peptidoglycan Biosynthesis (EC 6.3.2.13)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (485 amino acids)

>TX73_018320 UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2, 6-diaminopimelate ligase (Rhodopseudomonas palustris CGA009)
MKLRDIFSAEADIAAPAADLTIGGLAVDSRAVKPGDLFFALAGAKTDGARFVAAAVAAGA
VAVAGEHRPEGDLSVPFVQLSNARLALAQAAARFYPRQPEVIAAVTGTSGKTSVAAFTRQ
IWQRLGHAAASIGTIGLVTPTRSVYGSLTTPDPIALHRSLDEIAREGITHLALEASSHGL
DQFRLDGVRVAAGGFTNLSRDHMDYHPTVAHYLNAKLRLFRNLVQDGGAAVISADHDCSA
QVIEAAGARGLRLITIGRNGDPGAGIKLIDAAVEGFAQQLTVEHAGKRHRIKLPLVGEFQ
IENALVAAGLVIGTGGDAAASFAALEHLEGAPGRLDLVGARNGAPVFVDYAHKPDALAKT
LQALRPYAKRKLVVVFGAGGDRDSGKRPLMGAIAAENADVVIITDDNPRSEDPATIRAAI
LAAAPGAREIGDRAEAIRTAIGELQPGDALVIAGKGHETGQIVGDRVLHFSDHDAAKAAL
SASAA