Protein Info for TX73_018230 in Rhodopseudomonas palustris CGA009

Annotation: NAD-dependent DNA ligase LigA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01653: DNA_ligase_aden
  • amino acids 19 to 334 (316 residues), 406.2 bits, E=2.4e-125
• TIGR00575: DNA ligase, NAD-dependent
  • amino acids 27 to 709 (683 residues), 801.6 bits, E=2.7e-245
• PF03120: DNA_ligase_OB
  • amino acids 337 to 424 (88 residues), 103.3 bits, E=1.3e-33
• PF03119: DNA_ligase_ZBD
  • amino acids 434 to 461 (28 residues), 50.5 bits, see alignment (E = 3.7e-17)
• PF12826: HHH_2
  • amino acids 541 to 572 (32 residues), 37.4 bits, see alignment (E = 5.6e-13)
• PF00533: BRCT
  • amino acids 643 to 713 (71 residues), 60.8 bits, E=3e-20

Best Hits

Swiss-Prot: 100% identical to DNLJ_RHOPA: DNA ligase (ligA) from Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009)

KEGG orthology group: K01972, DNA ligase (NAD+) [EC: 6.5.1.2] (inferred from 98% identity to rpt:Rpal_4035)

Predicted SEED Role

"DNA ligase (EC 6.5.1.2)" in subsystem DNA Repair Base Excision (EC 6.5.1.2)

MetaCyc Pathways

• NAD salvage pathway II (PNC IV cycle) (4/5 steps found)
• NAD salvage pathway III (to nicotinamide riboside) (2/3 steps found)
• NAD salvage pathway I (PNC VI cycle) (4/7 steps found)
• NAD salvage (plants) (4/11 steps found)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.5.1.2

Protein Sequence (715 amino acids)

>TX73_018230 NAD-dependent DNA ligase LigA (Rhodopseudomonas palustris CGA009)
MTAKTKPAPDIATLTKPKAKVELMRLRLEIEGHDKAYYQDDAPKISDADYDALRRRLEAI
EQKFPELVNASSPTQTVGAAPARGFAKVQHAVPMLSLGNAFADDEVAEFALRVQRFLKLD
DVPAIVAEPKIDGLSLSLRYENGELVRAATRGDGFTGEDVTANVRTIKDVPNTLKGKHIP
ATCELRGEVYMLKQDFLALNKRQEEANETVFANPRNSAAGSLRQKDVTVTASRPLKFFAY
AWGEMSDYPMEEPTQHKMLQWLDHAGFVVNPEITLCHSVEDALAFYRRIGEKRASLPYDI
DGVVYKVDRLDYQERLGFVSRSPRWAIAHKFAAEQATTVLEKIDIQVGRTGAMTPVARLQ
PVTVGGVVVQNATLHNEDYIKGIGNDGEPIRDGVDIREGDTVVVQRAGDVIPQIVSVVME
KRPAGAEPYHFPHKCPVCGSHAVREEGEAVWRCTGALICPAQAVERLKHFVSRLAFDIDG
LGEKQIELFHERGWVQEPADIFTLKARNAELKLEQLEGYGETSVRNLFAAIDARRSIELH
RLVFALGIRHVGEGNAKLLARHYGTLDAFLSAMRAAADAQTEEGNTSEAYQDLDNIAGIG
DVVAEAVVEFFAEERNIKALDALLAELTEVLPAEQARRDTAVAGKTVVFTGSLSKFTRDE
AKAAAERLGAKVAGSVSKKTDYVVAGEDAGSKLTKAKDLGVTVLTEDEWLALIGN