Protein Info for TX73_017845 in Rhodopseudomonas palustris CGA009

Annotation: isobutyryl-CoA dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 382 PF02771: Acyl-CoA_dh_N" amino acids 7 to 118 (112 residues), 121.3 bits, see alignment E=5.4e-39 PF02770: Acyl-CoA_dh_M" amino acids 123 to 215 (93 residues), 88.7 bits, see alignment E=4.6e-29 PF00441: Acyl-CoA_dh_1" amino acids 230 to 378 (149 residues), 165.7 bits, see alignment E=1.8e-52 PF08028: Acyl-CoA_dh_2" amino acids 245 to 366 (122 residues), 78 bits, see alignment E=1.7e-25

Best Hits

Swiss-Prot: 60% identical to ACAD8_HUMAN: Isobutyryl-CoA dehydrogenase, mitochondrial (ACAD8) from Homo sapiens

KEGG orthology group: K00257, [EC: 1.3.99.-] (inferred from 100% identity to rpa:RPA3448)

MetaCyc: 43% identical to 5-carboxy-2-pentenoyl-CoA reductase (Thermobifida fusca B6)
RXN-22975

Predicted SEED Role

"Branched-chain acyl-CoA dehydrogenase (EC 1.3.99.12)" in subsystem Isobutyryl-CoA to Propionyl-CoA Module or Isoleucine degradation or Leucine Degradation and HMG-CoA Metabolism or Valine degradation (EC 1.3.99.12)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.3.99.-

Use Curated BLAST to search for 1.3.99.- or 1.3.99.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (382 amino acids)

>TX73_017845 isobutyryl-CoA dehydrogenase (Rhodopseudomonas palustris CGA009)
MDQFSLNEDQRAIRDMARDFAAEKIAPHALQWDEDKHLPLDVIREAAALGIGGIYIRDDV
GGSAMTRFDAALIFEALATGCPSVSAFISIHNMAAWMIDSYGSDAQRQRWLPKLCSMEQV
ASYCLTEPGSGSDAAALRTRAVRDGDHYILNGQKQFISGAGANDLYVVMVRTGSDGPGGI
STLVVEKDTPGLSFGANERKMGWNAQPTRTVIFENARVPVANRLGDEGIGFKIAMAGLDG
GRLNIGACSLGGAQAALDKTLAYMKERKAFGKRLDEFQALQFRLADMATELEAARTLLWR
AAAALDRKDADATKLCAMAKRFATDAGFGVANEALQLHGGYGYLAEYGIEKLVRDLRVHQ
ILEGTNEIMRLIVSRKLLEANR