Protein Info for TX73_017120 in Rhodopseudomonas palustris CGA009

Annotation: glycogen debranching protein GlgX

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 733 TIGR02100: glycogen debranching enzyme GlgX" amino acids 41 to 730 (690 residues), 1047.5 bits, see alignment E=0 PF02922: CBM_48" amino acids 45 to 130 (86 residues), 60.6 bits, see alignment E=1.5e-20 PF00128: Alpha-amylase" amino acids 225 to 313 (89 residues), 38.4 bits, see alignment E=1.1e-13

Best Hits

Swiss-Prot: 54% identical to GLGX_MYCBO: Glycogen operon protein GlgX homolog (glgX) from Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)

KEGG orthology group: K02438, glycogen operon protein GlgX [EC: 3.2.1.-] (inferred from 100% identity to rpa:RPA3311)

Predicted SEED Role

"Glycogen debranching enzyme (EC 3.2.1.-)" in subsystem Glycogen metabolism or Trehalose Biosynthesis (EC 3.2.1.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.-

Use Curated BLAST to search for 3.2.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (733 amino acids)

>TX73_017120 glycogen debranching protein GlgX (Rhodopseudomonas palustris CGA009)
MTDTNETLELPPETKPAEPAAKPQAAPGYNVFNDTKAVREGLPYPLGSCWDGHGTNFALF
SANATKVEVCLFDGDTERRIELPEYTDEVFHGYIPDIGPGTFYGYRVYGPYEPENGHRFN
PNKLLLDPYARAHAGLLTWDPAVFGYVMESGDDTTFDERDSARFVPKCVVVDPDFDWKGE
PGRKFVPWDQAIIYETHVKGFTKLHPDVPEHLRGTYAGFGTKPVVDYISSLGVTAVELLP
IHTFVNDSYLLDKNLVNYWGYNTIGFFAPDPRYAADVPNSLREFKEMVARLHDVGLAVIL
DVVYNHTAEGNELGPTLSFKGIDNASYYRLLPEQKRYYINDTGTGNTVNLTHPRVIQMVN
DSLRYWAGEMHIDGFRFDLGTILAREHYGFDEQSGFLKSVMQDPLLSSVKLIAEPWDCGP
GGYQVGGFPPGWAEWNDKFRDTARDYWRGEAPAAALAPRLSASGDVFNRQGRRAWASVNF
ITAHDGFTLNDWASYNDKHNEANGEDNRDGHSDNRSWNCGAEGPTDDETILSLRERQKRN
MLATLLLAQGTPMLLAGDEFGRTQNGNNNAYCQDNEISWVHWDHDEAAQHLRAFTQRLLE
LRRTTPTLRRSRFLTGQYDETLDVRDVTWINANGGEMQQEHWDDSNVKCFGMLLDGRAQT
TGIRRLGEEATVLLVLNGFDGMVEFTLPEVPHGTKWSLLIDTNLPHGVPDEEFECGAAYQ
VTARSVLLFKLGK