Protein Info for TX73_016490 in Rhodopseudomonas palustris CGA009

Annotation: PQQ-dependent dehydrogenase, methanol/ethanol family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 724 signal peptide" amino acids 1 to 44 (44 residues), see Phobius details TIGR03075: PQQ-dependent dehydrogenase, methanol/ethanol family" amino acids 58 to 590 (533 residues), 714.7 bits, see alignment E=3.4e-219 PF13570: PQQ_3" amino acids 104 to 143 (40 residues), 23.5 bits, see alignment 1.5e-08 amino acids 144 to 194 (51 residues), 22.6 bits, see alignment 3e-08 amino acids 515 to 554 (40 residues), 24.3 bits, see alignment 8.6e-09 PF01011: PQQ" amino acids 125 to 153 (29 residues), 23.8 bits, see alignment (E = 7e-09) amino acids 176 to 202 (27 residues), 27.1 bits, see alignment (E = 6.3e-10) amino acids 536 to 572 (37 residues), 33.2 bits, see alignment 7.7e-12 PF13360: PQQ_2" amino acids 134 to 262 (129 residues), 57.4 bits, see alignment E=4.4e-19 amino acids 504 to 571 (68 residues), 35.6 bits, see alignment E=2e-12 PF13442: Cytochrome_CBB3" amino acids 639 to 713 (75 residues), 45.1 bits, see alignment E=2.6e-15

Best Hits

Swiss-Prot: 69% identical to QHED_COMTE: Quinohemoprotein alcohol dehydrogenase (qheDH) from Comamonas testosteroni

KEGG orthology group: K00114, alcohol dehydrogenase (cytochrome c) [EC: 1.1.2.8] (inferred from 100% identity to rpa:RPA3188)

MetaCyc: 69% identical to alcohol dehydrogenase (azurin) (Comamonas testosteroni)
RXN-11335 [EC: 1.1.9.1]

Predicted SEED Role

"Quino(hemo)protein alcohol dehydrogenase, PQQ-dependent (EC 1.1.99.8)" (EC 1.1.99.8)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.2.8 or 1.1.9.1 or 1.1.99.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (724 amino acids)

>TX73_016490 PQQ-dependent dehydrogenase, methanol/ethanol family (Rhodopseudomonas palustris CGA009)
MRKPADAVRTAIRPVQQPAIRLVARRLGAAALMAALLTPAVAAAQDSKGSAAHIRAVTGA
VDSAAIVANVKTTKDWPSYGLDYAETRFSKLDQINTENVKQLGLQWSYSLGSERGVEATP
VVVDGIMYVTASWSVVHAIDTRTGKKLWTFDPKVDHSKGYRGCCDVVNRGVALYKGKVFV
GAYDGRLIALDAATGSKAWEIDTLIDHEHSYTITGAPRVFNGKVVIGNGGAEYGARGYVT
AYDAETGKQAWRWFTVPGDPSKPFEDESMEKAAKTWDPAGKWWLNGGGGTAWDTITFDPD
LNLIYVGTGNGSPWNRHLRSPAGGDNLYLASIVALNADTGKYVWHYQETPGDNWDYTSTQ
PMILADIAIDGKPRKVILHAPKNGFFFVIDRTDGKFISAKNFVDVNWATGYDSNGRPIEV
PEARSADKSFDAIPGPYGAHNWHPMSFNPQTGLVYLPAQGVPVNLTGEKALTQNKMEPFK
FGSTTGWNVGFTLNAVPPKNLPFGRLVAWDPVQQKEVWRAEYVSPWNGGTLTTAGNLVFQ
GTADGRFVAYNAKTGEKLWESPLGTGAVAAPATYMVDGVQYVSIAVGWGGVFGISARATE
TEAPGTVYTFAVGGKAKMPEFAKYQMGNLLTGIEYDPKDVAEGTAIYVAACATCHGVPGV
DRGGNVKNLGYSTTDRIAHLKDIVFKGPFRDKGMPDFTGKLTEADVVKIQAFIQGTADAI
RPKK