Protein Info for TX73_014840 in Rhodopseudomonas palustris CGA009

Annotation: pyruvate dehydrogenase complex dihydrolipoamide acetyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01349: pyruvate dehydrogenase complex dihydrolipoamide acetyltransferase
  • amino acids 4 to 463 (460 residues), 567.5 bits, E=1.2e-174
• PF00364: Biotin_lipoyl
  • amino acids 5 to 77 (73 residues), 70.1 bits, E=1.7e-23
• PF02817: E3_binding
  • amino acids 159 to 194 (36 residues), 60.4 bits, 2.4e-20
• PF00198: 2-oxoacid_dh
  • amino acids 230 to 462 (233 residues), 274.3 bits, E=1.2e-85

Best Hits

KEGG orthology group: K00627, pyruvate dehydrogenase E2 component (dihydrolipoamide acetyltransferase) [EC: 2.3.1.12] (inferred from 100% identity to rpa:RPA2864)

Predicted SEED Role

"Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex (EC 2.3.1.12)" in subsystem Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate (EC 2.3.1.12)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (23/26 steps found)
• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (19/22 steps found)
• 1-butanol autotrophic biosynthesis (engineered) (21/27 steps found)
• photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (20/26 steps found)
• pyruvate decarboxylation to acetyl CoA I (3/3 steps found)
• pyruvate fermentation to acetate II (3/3 steps found)
• pyruvate fermentation to acetate V (3/3 steps found)
• anaerobic energy metabolism (invertebrates, mitochondrial) (8/10 steps found)
• superpathway of anaerobic energy metabolism (invertebrates) (13/17 steps found)
• pyruvate fermentation to acetate and (S)-lactate I (3/4 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Citrate cycle (TCA cycle)
• Glycolysis / Gluconeogenesis
• Pyruvate metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.1.12

Protein Sequence (463 amino acids)

>TX73_014840 pyruvate dehydrogenase complex dihydrolipoamide acetyltransferase (Rhodopseudomonas palustris CGA009)
MPINILMPALSPTMEKGNLAKWLKKEGDKVKSGDVIAEIETDKATMEVEAADEGTLAKII
VPEGTQDVPVNDVIAVLAADGEDVKAAGAGWKASAGGAPSPQRGEGAGPSGGKAEANSHI
QDKADQRPTPQPPSPLPNGERSLPQAAGEGAPAPANGRVFASPLARRLAKDAGIDIARVT
GTGPHGRVIARDVEQAKSGGGLKAPASAPAGPAIAAAMSDQQIRALYPEGSYEVVPHDGM
RRTIAQRLTQSTQTIPHFYLTIDCNLDRLLAAREDINAAAPKDKDGKPAYKLSVNDFIIK
AMAIALQRIPDANVSWTEGGMLKHKHSDIGVAVAMPGGLITPIIRSAETQSLSSISAQMK
DFAARARARKLKPEEYQGGTTAVSNLGMFGIKDFTAVINPPHATILAVGTGEQRAIVKDG
KIEVATMMSVTLSCDHRAVDGALGAELIGAFKTLIENPVMMMV