Protein Info for TX73_013955 in Rhodopseudomonas palustris CGA009

Annotation: bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 760 TIGR00691: RelA/SpoT family protein" amino acids 60 to 756 (697 residues), 650.6 bits, see alignment E=1.4e-199 PF13328: HD_4" amino acids 60 to 209 (150 residues), 169.9 bits, see alignment E=1.2e-53 PF01966: HD" amino acids 79 to 178 (100 residues), 41.6 bits, see alignment E=4.8e-14 PF04607: RelA_SpoT" amino acids 269 to 377 (109 residues), 135.1 bits, see alignment E=4.3e-43 PF02824: TGS" amino acids 426 to 484 (59 residues), 62.3 bits, see alignment 1.2e-20 PF19296: RelA_AH_RIS" amino acids 496 to 668 (173 residues), 223.2 bits, see alignment E=1.1e-69 PF13291: ACT_4" amino acids 681 to 757 (77 residues), 58.2 bits, see alignment E=3.8e-19 PF01842: ACT" amino acids 686 to 748 (63 residues), 31.6 bits, see alignment E=3.9e-11

Best Hits

KEGG orthology group: K00951, GTP pyrophosphokinase [EC: 2.7.6.5] (inferred from 100% identity to rpa:RPA2693)

Predicted SEED Role

"GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase II / Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (EC 3.1.7.2)" in subsystem Stringent Response, (p)ppGpp metabolism (EC 2.7.6.5, EC 3.1.7.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.6.5 or 3.1.7.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (760 amino acids)

>TX73_013955 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (Rhodopseudomonas palustris CGA009)
MASGRQGSKQMEQAGGAAAVAEPTAVAKPRSRARMMRQYDLVERVRSYNPNTDEDLLNRA
YVYAMMAHGEQTRASGDPYFSHPLEVAAILTNLKLDDATIVAALLHDTIEDTEATRTEID
TMFGPEIGALVEGLTKLKRLELVSREAKQAENLRKLLLAIADDVRVLLIKLADRLHNMRT
LEFVPPASRQRIAEETLDIYAPLAGRMGMQEMREELEDLSFRSLDPEAFTVVMQRLDALA
ERNRNLIGEIESQLSANLARHGITAEVTGRRKRPFSIWTKMERKSVGFEQLSDIYGFRVV
LDNVEACYRALGVVHTTWPMVPGRFKDYISTPKQNDYRSLHTTVIGPGQQRVELQFRTRE
MNQIAEYGIAAHAFYKDGVGSPTELLNRESNAFAWLRHTIEMLSESSNPEEFLEHTKLEL
FHDQVFCFTPKGKLIALPRKANVVDFAYAVHTDVGNSAVGCKINGKFAPLSSELQNGDEV
EVLTSPVQSAPPAAWESLAVTGKARAAIRRATRVAMRDQYAGLGRRIVERLFARAKIEYA
DDKLKGALPRLARASVEDVMASVGRGEMKASDVARAMYPDYKEERIARFGGKKDVPDKVI
GKAPETGKVSSVIPIGGLHSGLPVKFAPNGGAVPGDRIVGIVTPGEGITIYPIQSPDLKE
FEEEPERWLDVKWDIDETTPQRFPARLFVQNVNEPGSLAQIAGVIAEHDGNIDNINMSRR
SPDFTELTIDLEVYDLKHLSAIIAQLRAKDVVAHVERVNG