Protein Info for TX73_013480 in Rhodopseudomonas palustris CGA009

Annotation: tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 360 transmembrane" amino acids 317 to 335 (19 residues), see Phobius details TIGR00113: S-adenosylmethionine:tRNA ribosyltransferase-isomerase" amino acids 1 to 353 (353 residues), 348.3 bits, see alignment E=2e-108 PF02547: Queuosine_synth" amino acids 4 to 353 (350 residues), 398.9 bits, see alignment E=8.5e-124

Best Hits

Swiss-Prot: 100% identical to QUEA_RHOPA: S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) from Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009)

KEGG orthology group: K07568, S-adenosylmethionine:tRNA ribosyltransferase-isomerase [EC: 5.-.-.-] (inferred from 100% identity to rpa:RPA2605)

Predicted SEED Role

"S-adenosylmethionine:tRNA ribosyltransferase-isomerase (EC 5.-.-.-)" in subsystem Queuosine-Archaeosine Biosynthesis (EC 5.-.-.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 5.-.-.-

Use Curated BLAST to search for 5.-.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (360 amino acids)

>TX73_013480 tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA (Rhodopseudomonas palustris CGA009)
MRTELFDFDLPAENIALRPVSPRDAARMLVVRPGAPLEDRIVRDLPALLAPGDQLVVNDT
RVIAAQLTGRRIGRGLEPKIEATLIKRLDGARWQALVKPAKKLLPGDVVRFGHDGRVCLL
GHLDATVEAKGDAGEVTLVFSFHGPVLDQAIAEVGATPLPPYIASKRAPDAQDIADYQTM
FASNEGAVAAPTAGLHFTAELETALAACGVGLHRITLHVGAGTFLPVKAEDTSEHRMHAE
WGTISSGTAEALNAARAAGGRIVAVGTTSLRLLESAARDDGRIAPFADETSIFITPGYRF
RAVDVLMTNFHLPRSTLFMLVSAFCGLDTMQAAYAHAIRTGYRFYSYGDASLLFRNDITP