Protein Info for TX73_013450 in Rhodopseudomonas palustris CGA009

Annotation: DNA gyrase subunit A

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 913 TIGR01063: DNA gyrase, A subunit" amino acids 18 to 902 (885 residues), 1177.7 bits, see alignment E=0 PF00521: DNA_topoisoIV" amino acids 42 to 507 (466 residues), 564 bits, see alignment E=2.3e-173 PF03989: DNA_gyraseA_C" amino acids 539 to 585 (47 residues), 52.4 bits, see alignment 2.9e-18 amino acids 591 to 638 (48 residues), 20.9 bits, see alignment 1.9e-08 amino acids 649 to 692 (44 residues), 35.7 bits, see alignment 4.6e-13 amino acids 696 to 742 (47 residues), 29.4 bits, see alignment 4.2e-11 amino acids 805 to 851 (47 residues), 42.1 bits, see alignment 4.6e-15 amino acids 855 to 901 (47 residues), 48.2 bits, see alignment 5.7e-17

Best Hits

Swiss-Prot: 57% identical to GYRA_GRABC: DNA gyrase subunit A (gyrA) from Granulibacter bethesdensis (strain ATCC BAA-1260 / CGDNIH1)

KEGG orthology group: K02469, DNA gyrase subunit A [EC: 5.99.1.3] (inferred from 88% identity to bra:BRADO3999)

Predicted SEED Role

"DNA gyrase subunit A (EC 5.99.1.3)" in subsystem DNA topoisomerases, Type II, ATP-dependent or Resistance to fluoroquinolones (EC 5.99.1.3)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.3

Use Curated BLAST to search for 5.99.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (913 amino acids)

>TX73_013450 DNA gyrase subunit A (Rhodopseudomonas palustris CGA009)
MSDKDDEKPEGPQAPSDIRPVSILDEMKRSYLDYAMSVIVARALPDARDGLKPVHRRILY
GMYENGFEWNKQYRKSARTVGDVIGKYHPHGDQSVYDALVRMAQDFSMRVPLIDGQGNFG
SVDGDMPAAMRYTESRLTKIAQTLLDDIDKDTVDFQPNYDNSEREPQVLPAKFPNLLVNG
AGGIAVGMATNIPPHNLGEVIDACIALIDDPALSIDDLNKIVPGPDFPTGGIILGRAGIR
AAYQTGRGSIVMRGKVEIETVRKDREAIIVSEIPYQVNKATMVERIAELVREKKIEGIGD
LRDESDRDGFRVVIELRRDAVPEVVLNQLYKFTPLQTNFGANMVALEGGRPQLMNLKDLL
TVFVAFREQVVTRRTKFLLNKARDRAHILVGLAIAVANIDEIIRVIRNSPDPNTARETLM
SRDWPAADVAAMITLIDDPRHKLNPDGTARLSFEQAKAILDLRLQRLTALGREEISEELD
KLAVEIADYLEILRSRARVQAIVKTELGEVKAEFATPRRTVIAEQEGEVEDEDLIQREDM
VVTVSHAGYVKRVPLSTYRAQRRGGKGRSGMATREEDFVSRLFVASTHTPVLFFSSRGQV
YKEKVWRLPLAPPNGRGKALINILPLEQGERITTIMPLPEDEASWSELDVMFATTGGNVR
RNKLSDFVDVRRSGIIAMKLDEGEAIVDVQICTERDDVLLTAAGGQCIRFPVPDVRVFSG
RTSMGVRGIALSSGDTVISLSILRHFEATPAERSTYLKQSGAIRRAATGEENEPVETPEA
EAEEGDTSAALSQERYAEMSASEQFVLTISENGYGKRTSSFEYRTTGRGGKGIVAMSVNS
RNGKLVASFPVEDSDQIMLVTDNGQLIRCPVEGIRVAGRSTQGVIVFNTADDEKVVSVER
IPETDDGENGNGG