Protein Info for TX73_013425 in Rhodopseudomonas palustris CGA009

Annotation: HAMP domain-containing histidine kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 763 transmembrane" amino acids 27 to 51 (25 residues), see Phobius details amino acids 59 to 79 (21 residues), see Phobius details amino acids 100 to 122 (23 residues), see Phobius details amino acids 298 to 325 (28 residues), see Phobius details amino acids 395 to 416 (22 residues), see Phobius details PF19312: NtrY_N" amino acids 33 to 309 (277 residues), 119.6 bits, see alignment E=5.2e-38 PF00672: HAMP" amino acids 322 to 373 (52 residues), 41.5 bits, see alignment 4.1e-14 TIGR00229: PAS domain S-box protein" amino acids 391 to 504 (114 residues), 40.5 bits, see alignment E=1.4e-14 PF00989: PAS" amino acids 392 to 482 (91 residues), 40.7 bits, see alignment E=6.6e-14 PF00512: HisKA" amino acids 509 to 575 (67 residues), 41 bits, see alignment E=4.9e-14 PF02518: HATPase_c" amino acids 619 to 732 (114 residues), 66.1 bits, see alignment E=1.1e-21

Best Hits

Swiss-Prot: 58% identical to NTRY_AZOC5: Nitrogen regulation protein NtrY (ntrY) from Azorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / JCM 20966 / NBRC 14845 / NCIMB 13405 / ORS 571)

KEGG orthology group: K13598, two-component system, NtrC family, nitrogen regulation sensor histidine kinase NtrY [EC: 2.7.13.3] (inferred from 100% identity to rpa:RPA2594)

Predicted SEED Role

"Nitrogen regulation protein NtrY (EC 2.7.3.-)" (EC 2.7.3.-)

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3, 2.7.3.-

Use Curated BLAST to search for 2.7.13.3 or 2.7.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (763 amino acids)

>TX73_013425 HAMP domain-containing histidine kinase (Rhodopseudomonas palustris CGA009)
MTSAETSAQPVDDPSTAEPRLRTWRKLLAPMAVGVALFSAFLTFIVLTGLTPIAPTREVV
VSFLLINAVTILLLLAIIAREVWKVVQARRRGRAASRLHIQIVSLFSVIAVLPAVLVSIV
ANVTLDRGLDRLFSGPTRAVIQNSLIVANAYLHEHGQLIRGDILGMANDISHARALFDLD
RKSFREFLTNDAATRNLPGAMLIDKDRNILEAAQNGIRRDFDVPAPEFLKNVDDTEPQIA
VFIEANYVAAVIRLKGFEDTFLYVARFLDPHVVAQLRQTQASVAEYSELEARRLGVQVAF
ALMFTVIALTVLMSAVLIGLNFANWLVAPIRRLMGAAHLVSTGDLHVQVPVSKSEGDLSQ
LGMTFNKMTQELRTQRDELVSASELIDSRRRFIEAVLSSASAGIIGVDGSLSIGILNRSA
EKLIGHAEAEALGHPLSEVIPELDEIMGTAREGSQRLVQGQITIVRDGRERILNVRVTAE
QTGHTSDSYIITLDDITELVSAQRTSAWGDVARRIAHEIKNPLTPIQLSAERIKRKFGKV
IVEDKNIFEQCTDTIVRQVDDIRRMVDEFSRFARMPKPVIEGEDVGDTVRQVVFLMRVGH
PDVDIEAEIKDEPLHARFDRRLISQALTNIIKNATEAIEAVPEEELGKGKIEVVAAREGD
DVVIDVIDNGIGLPKESRSRLLEPYVTTREKGTGLGLAIVGRVLVDHGGGIELNDASHVR
PGQRGAWMRLRFAMSGQPASPPGSGEPKQQAENEPRIAAASGS