Protein Info for TX73_013405 in Rhodopseudomonas palustris CGA009

Annotation: bifunctional 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase/2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01128: IspD
  • amino acids 8 to 225 (218 residues), 202.6 bits, E=1.1e-63
• PF12804: NTP_transf_3
  • amino acids 9 to 138 (130 residues), 30.9 bits, E=4.3e-11
• TIGR00453: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
  • amino acids 9 to 223 (215 residues), 226 bits, E=3.9e-71
• PF02542: YgbB
  • amino acids 234 to 387 (154 residues), 208.5 bits, E=1e-65
• TIGR00151: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase
  • amino acids 235 to 387 (153 residues), 199.9 bits, E=2.4e-63

Best Hits

Swiss-Prot: 100% identical to ISPDF_RHOPA: Bifunctional enzyme IspD/IspF (ispDF) from Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009)

KEGG orthology group: K12506, 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase / 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase [EC: 2.7.7.60 4.6.1.12] (inferred from 100% identity to rpa:RPA2590)

Predicted SEED Role

"2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (EC 2.7.7.60) / 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (EC 4.6.1.12)" in subsystem Isoprenoid Biosynthesis or polyprenyl synthesis or Teichoic and lipoteichoic acids biosynthesis (EC 2.7.7.60, EC 4.6.1.12)

MetaCyc Pathways

• methylerythritol phosphate pathway II (8/9 steps found)
• superpathway of geranylgeranyl diphosphate biosynthesis II (via MEP) (10/12 steps found)
• taxadiene biosynthesis (engineered) (10/13 steps found)
• methylerythritol phosphate pathway I (7/9 steps found)
• isoprene biosynthesis I (7/10 steps found)
• superpathway of ergosterol biosynthesis II (11/26 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Terpenoid biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.60 or 4.6.1.12

Protein Sequence (398 amino acids)

>TX73_013405 bifunctional 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase/2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (Rhodopseudomonas palustris CGA009)
MTNSPRTAAIIVAAGRGLRAGAGGPKQYRSLAGRPVIARAMEPFCTHPGVMAVQPVTNPD
DTEMFNAAVAGLNFRPAVGGGATRQASVRAGLEALAELKPDIVLIHDAARCFVTPELISR
AITAAGATGAALPVVPVTDTIKQVDSSGAVDATPDRASLRIAQTPQAFRFDVILDAHRRA
ASGGRDDFTDDAAIAEWAGLTVSTFEGDANNMKMTTPEDFAREESRLMAALGDIRTGTGY
DVHAFGEGDHVWLCGLKVPHTRGFLAHSDGDVGLHALVDAILGALADGDIGSHFPPTDPQ
WKGAASDKFLKYAIDRVTARGGRVANLEVTMICERPKIGPLRDAMRQRIAEITGVPVSRV
AVKATTSEKLGFTGREEGIAATASATIRLPWGADGLAG