Protein Info for TX73_010735 in Rhodopseudomonas palustris CGA009

Annotation: cobyrinate a,c-diamide synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 453 PF01656: CbiA" amino acids 19 to 196 (178 residues), 52.2 bits, see alignment E=9.1e-18 TIGR00379: cobyrinic acid a,c-diamide synthase" amino acids 20 to 452 (433 residues), 314 bits, see alignment E=8.7e-98 PF13500: AAA_26" amino acids 118 to 199 (82 residues), 29.6 bits, see alignment E=9.3e-11 PF07685: GATase_3" amino acids 259 to 445 (187 residues), 98.6 bits, see alignment E=5.8e-32

Best Hits

Swiss-Prot: 54% identical to COBB_RHILO: Hydrogenobyrinate a,c-diamide synthase (cobB) from Mesorhizobium japonicum (strain LMG 29417 / CECT 9101 / MAFF 303099)

KEGG orthology group: K02224, cobyrinic acid a,c-diamide synthase [EC: 6.3.1.- 6.3.5.9] (inferred from 100% identity to rpa:RPA2083)

Predicted SEED Role

"Cobyrinic acid A,C-diamide synthase" in subsystem Cobalamin synthesis or Coenzyme B12 biosynthesis

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.1.- or 6.3.5.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (453 amino acids)

>TX73_010735 cobyrinate a,c-diamide synthase (Rhodopseudomonas palustris CGA009)
MTVTDHILPTATNGRPPGLIIAAPSTNSGKTTVTLALHAALRRRSLTVQPYKCGPDYIDP
AFHEIAAGRPCYNLDSWAFGQDRITYLLDAAAGADLCLAEGVMGLFDGGSARGAWGNGAT
ADIAAFTGWPVVLVIDVAGQAQSAAALALGFARYRSDVRIAGVILNNVASARHEALVRDG
FAAQDIPVLGGFERQRDLTLKNRHLGLVQARETARLDIRLQQLADSAVRGIDLDALIRAA
QPARPIASTIRPLTPPGQRIALAHDDAFSFIYPHLTAGWRAAGAEIVPFSPLNDEGPDPS
CDAAWLPGGYPELHAGRLAAATRFKAALGAFAETRAVHGECGGYMTLGAGLIDADGTRHA
MAGLLGLETDFASRKLHLGYRLATLHADAAGKAAGSVLRGHEYHYARVVTVADEPLAEIR
DPAGQLTDETGSRRGHVTGTFFHLIDVAEEAIA