Protein Info for TX73_010460 in Rhodopseudomonas palustris CGA009

Annotation: tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00174: tRNA dimethylallyltransferase
  • amino acids 16 to 302 (287 residues), 251.5 bits, E=5.1e-79
• PF01745: IPT
  • amino acids 16 to 73 (58 residues), 34 bits, E=3.1e-12
• PF01715: IPPT
  • amino acids 48 to 292 (245 residues), 263.9 bits, E=2.3e-82

Best Hits

Swiss-Prot: 100% identical to MIAA_RHOPA: tRNA dimethylallyltransferase (miaA) from Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009)

KEGG orthology group: K00791, tRNA dimethylallyltransferase [EC: 2.5.1.75] (inferred from 100% identity to rpa:RPA2030)

Predicted SEED Role

"tRNA dimethylallyltransferase (EC 2.5.1.75)" (EC 2.5.1.75)

MetaCyc Pathways

• cis-zeatin biosynthesis (1/5 steps found)

KEGG Metabolic Maps

• Biosynthesis of plant hormones
• Zeatin biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.5.1.75

Protein Sequence (316 amino acids)

>TX73_010460 tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA (Rhodopseudomonas palustris CGA009)
MTAEGPERHGRRPAAVLIAGPTASGKSALALKLAQASGGTVINTDSMQVYRDLRIITARP
TPDEEALAPHRLYGTVDAAVNFSAGAYVEAAAAALAEVRAAGRLPILIGGTGLYFKALTR
GLSAVPPVPAEVRDAVRLRLDRDGVQALHAELARQDPEAAARLAPADRTRIARALEVVLA
TGRPLADWHQQTSPPLLPPDDVVAVFLAPDREALYARIDSRFAAMLQGGALDEVAALAER
QLDPLLPAMKAHGVPALIRHLRGEITLEEAASIGAADTRHYAKRQFTWFRHQLPEFKWVR
PEEADAFLNTVIPGRA