Protein Info for TX73_010310 in Rhodopseudomonas palustris CGA009

Annotation: citramalate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 532 TIGR00977: citramalate synthase" amino acids 6 to 528 (523 residues), 624.8 bits, see alignment E=4.9e-192 PF00682: HMGL-like" amino acids 6 to 256 (251 residues), 161.4 bits, see alignment E=3.3e-51 PF08502: LeuA_dimer" amino acids 383 to 523 (141 residues), 96.3 bits, see alignment E=1.4e-31

Best Hits

KEGG orthology group: K01649, 2-isopropylmalate synthase [EC: 2.3.3.13] (inferred from 100% identity to rpt:Rpal_2211)

Predicted SEED Role

"(R)-citramalate synthase (EC 2.3.1.182)" in subsystem Branched-Chain Amino Acid Biosynthesis (EC 2.3.1.182)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.3.3.13

Use Curated BLAST to search for 2.3.1.182 or 2.3.3.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (532 amino acids)

>TX73_010310 citramalate synthase (Rhodopseudomonas palustris CGA009)
MSRERLYLYDTTLRDGAQTNGVDFTLHDKKLIASLLDDLGIDYVEGGYPGANPLDTEFFG
TEQKLERATFAAFGMTRRPGRSASNDPGIAMLIDAKADAICFVAKSSEYQVRVALETTNE
ENIASIRDSVSLAKDKGREVLVDCEHFFDGYKANPEFALQCAKAAYESGARWVVLCDTNG
GTMPDEVEAIVGEVVKHIPGAHVGIHAHNDTEQAVANSFAAVRAGARQIQGTLNGLGERC
GNANLCSMIPTLKLKKEFADKFEIGVSDDKLASLVQVSRALDNILDRAPNPHAPYVGGSA
FVTKTGIHASAVLKDPQTYEHVPPESIGNHRKVLVSDQAGKSNVLAELARTNIQFDRDDP
RLSRLVEKLKEREAAGYAYESANASFDLLARSTLGTVPEFFRVDKFDVNVEQRYNSHGQR
VTVAMAVIKVEVDGETLISAAEGNGPVNALDVALRKDLGKYQKYIEGLKLVDYRVRILNG
GTEAVTRVLIESEDEDGRRWTTIGVSPNIIDASFQALMDSVVYKLVQSNAPV