Protein Info for TX73_010235 in Rhodopseudomonas palustris CGA009

Annotation: NAD+ synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00795: CN_hydrolase
  • amino acids 9 to 248 (240 residues), 90.9 bits, E=9e-30
• PF02540: NAD_synthase
  • amino acids 278 to 533 (256 residues), 232.1 bits, E=5.6e-73
• TIGR00552: NAD+ synthetase
  • amino acids 283 to 540 (258 residues), 192.2 bits, E=4.7e-61

Best Hits

KEGG orthology group: K01916, NAD+ synthase [EC: 6.3.1.5] (inferred from 100% identity to rpa:RPA1986)

Predicted SEED Role

"NAD synthetase (EC 6.3.1.5) / Glutamine amidotransferase chain of NAD synthetase" in subsystem NAD and NADP cofactor biosynthesis global or NAD regulation (EC 6.3.1.5)

MetaCyc Pathways

• aspartate superpathway (24/25 steps found)
• NAD de novo biosynthesis I (6/6 steps found)
• NAD biosynthesis from 2-amino-3-carboxymuconate semialdehyde (4/4 steps found)
• NAD de novo biosynthesis III (5/6 steps found)
• NAD de novo biosynthesis IV (anaerobic) (5/6 steps found)
• NAD salvage pathway II (PNC IV cycle) (4/5 steps found)
• NAD salvage pathway I (PNC VI cycle) (4/7 steps found)
• NAD salvage pathway V (PNC V cycle) (2/5 steps found)
• NAD de novo biosynthesis II (from tryptophan) (4/9 steps found)
• NAD salvage (plants) (4/11 steps found)
• superpathway of NAD biosynthesis in eukaryotes (4/14 steps found)

KEGG Metabolic Maps

• Nicotinate and nicotinamide metabolism
• Nitrogen metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.1.5

Protein Sequence (584 amino acids)

>TX73_010235 NAD+ synthase (Rhodopseudomonas palustris CGA009)
MTEAARLTISLAQLNPTVGDIAGNADKARAARRRAAADGADLVVYPELFIAGYPPEDLVL
KPAFQAACRAAIEDLARETADGGPAMLIGSPWVDNGKLYNACALLDGGRIAAIRHKVNLP
NYGVFDEKRVFARGPVSGPVTIRGVRIGVPICEDTWLEESEDYENVVECLAETGAELLVV
PNGSPYARGKNDMRMSVSVARVTESDLPLVYVNQVGGQDELVFDGASFVLNADRTLAAQL
PGFVESVTTLSFVKGAAGWRCDGPVAPVIEGDEADYAACVLGLRDYVRKNGFPGVLLGIS
GGIDSALCAAIAVDALGADKVRGVMLPFRYTAQISLDDAGRLASALGFGYEVLPIAQAVE
GFEAILAKPFAGLERDITEENLQARTRGTLLMAISNKTGAMVVTTGNKSEMSVGYATLYG
DMNGGFNPIKDIYKTQVFRLSSLRNRWKPDDALGPDGEVIPESIIIRPPTAELRENQTDQ
DSLPPYDVLDAILERLVEREEPLASIVADGFDKDTVVRIDRLLNIAEYKRRQAAPGVKVT
RKNFGRDRRYPITNRFRDKAEVLPAPDESLVTKAGRASSEAFEG