Protein Info for TX73_010055 in Rhodopseudomonas palustris CGA009

Annotation: pyrroloquinoline quinone biosynthesis protein PqqE

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 392 TIGR02109: coenzyme PQQ biosynthesis enzyme PqqE" amino acids 26 to 383 (358 residues), 576.9 bits, see alignment E=1.5e-177 PF04055: Radical_SAM" amino acids 36 to 192 (157 residues), 95.3 bits, see alignment E=7.1e-31 PF13353: Fer4_12" amino acids 39 to 111 (73 residues), 24.6 bits, see alignment E=4.1e-09 PF13186: SPASM" amino acids 264 to 327 (64 residues), 26.6 bits, see alignment E=9.1e-10 TIGR04085: radical SAM additional 4Fe4S-binding SPASM domain" amino acids 264 to 355 (92 residues), 35.6 bits, see alignment E=1e-12

Best Hits

Swiss-Prot: 100% identical to PQQE_RHOPA: PqqA peptide cyclase (pqqE) from Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009)

KEGG orthology group: K06139, pyrroloquinoline quinone biosynthesis protein E (inferred from 100% identity to rpa:RPA1950)

MetaCyc: 47% identical to glutamate Cgamma--tyrosine C3 ligase (Klebsiella pneumoniae)
RXN-11176 [EC: 1.21.98.4]

Predicted SEED Role

"Coenzyme PQQ synthesis protein E" in subsystem Coenzyme PQQ synthesis or Pyrroloquinoline Quinone biosynthesis

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.21.98.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (392 amino acids)

>TX73_010055 pyrroloquinoline quinone biosynthesis protein PqqE (Rhodopseudomonas palustris CGA009)
MTEIVTSASVADGVAVLEKSGSVAETFGIPLAVLLELTHRCPLQCPYCSNPLELERGGAE
LSTDEWKRVLSELARIGVLQVHFSGGEPTARKDLVELVRHATEVGLYTNLITSAVLLSRE
RLAALADAGLAHVQISFQGSEATIADRVGGFAGGHAKKLDVARWTRELDLPLTVNAVMHR
QNLHLLPDIIELALALDADRLEVANVQYYGWALKNRAALMPTLAQIEDCTATVEAARERL
KGQLAIDYVIPDYYAARPKKCMGGWGRQFFNISPSGKVLPCHAAETITGLDFPSVRNGAS
IAEIWRSAEAFNRYRGTGWMQQPCASCAFKEIDFGGCRCQAFALAGDAAATDPACALSPL
HDRIFKTAEAEAASGSDRFLYRNFAGGTVEGA