Protein Info for TX73_009900 in Rhodopseudomonas palustris CGA009

Annotation: heme lyase CcmF/NrfE family subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 660 transmembrane" amino acids 6 to 27 (22 residues), see Phobius details amino acids 41 to 62 (22 residues), see Phobius details amino acids 96 to 114 (19 residues), see Phobius details amino acids 125 to 144 (20 residues), see Phobius details amino acids 175 to 195 (21 residues), see Phobius details amino acids 207 to 229 (23 residues), see Phobius details amino acids 249 to 265 (17 residues), see Phobius details amino acids 273 to 293 (21 residues), see Phobius details amino acids 312 to 331 (20 residues), see Phobius details amino acids 352 to 374 (23 residues), see Phobius details amino acids 393 to 413 (21 residues), see Phobius details amino acids 425 to 443 (19 residues), see Phobius details amino acids 449 to 469 (21 residues), see Phobius details amino acids 493 to 511 (19 residues), see Phobius details amino acids 618 to 637 (20 residues), see Phobius details TIGR00353: cytochrome c-type biogenesis protein CcmF" amino acids 53 to 643 (591 residues), 622.6 bits, see alignment E=4.4e-191 PF01578: Cytochrom_C_asm" amino acids 89 to 295 (207 residues), 179.5 bits, see alignment E=7.1e-57 PF16327: CcmF_C" amino acids 315 to 640 (326 residues), 402 bits, see alignment E=2.1e-124

Best Hits

Swiss-Prot: 81% identical to CCMF_BRADU: Cytochrome c-type biogenesis protein CycK (cycK) from Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)

KEGG orthology group: K02198, cytochrome c-type biogenesis protein CcmF (inferred from 100% identity to rpt:Rpal_2132)

Predicted SEED Role

"Cytochrome c heme lyase subunit CcmF" in subsystem Biogenesis of c-type cytochromes or Copper homeostasis

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (660 amino acids)

>TX73_009900 heme lyase CcmF/NrfE family subunit (Rhodopseudomonas palustris CGA009)
MIAEAGHYALVLALALALIQSTVPLLGARLNDGALMNVARSTALVQLLFAGLSFTALVWL
HVTSDFSVVNVYENSHSMKPLLYKITGVWGNHEGSMLLWVAILALFGGLVAAFGNNLPLS
LRAHVLAVQGWVATAFYLFILITSNPFARIANAPAEGRDLNPVLQDIGLAVHPPMLYLGY
VGFSISFSFAAAALMEGRLDAAWARWVRPWTLVAWIFLTLGIAMGSYWAYYELGWGGWWF
WDPVENASLMPWLAGTALLHSALVMEKRNALKVWTILLSILTFSLSLLGTFLVRSGVLTS
VHAFATDPSRGVFILIILCVFIGGSLTLFAWRASSLKQGGLFAPISREGSLVLNNLFLTT
ACATVFVGTLYPLALEVLTGDKISVGAPFFNLTFGPLMVPLLVAVPFGPLLAWKRGDLVG
AAQRLIAAGVVALLAVAFVYAWVHGGAVLAPLAIGLAVFVIIGALVDIAERIALFRGPLS
ISLRRASGLPRSAWGTMFAHAALGVTLIGIVCETTWNSEYIAAMKPGDSTKLAGYEFKFE
GANQRQGPNYRELLTQFTVTENGRLVGGMAPSKRSFITRGSSTTEAALLTRGFSQLYISL
GDLNDQGAVTVRIYHKPMVLLIWFGPILMALGGLLSLSDRRLRVGAPKPAKAPRVLQPAE