Protein Info for TX73_008865 in Rhodopseudomonas palustris CGA009

Annotation: phenylacetate--CoA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02155: phenylacetate-CoA ligase
  • amino acids 21 to 440 (420 residues), 742.1 bits, E=7e-228
• PF00501: AMP-binding
  • amino acids 91 to 299 (209 residues), 57.4 bits, E=1.2e-19
• PF14535: AMP-binding_C_2
  • amino acids 346 to 440 (95 residues), 83 bits, E=1.6e-27

Best Hits

Swiss-Prot: 65% identical to PAAK_AZOEV: Phenylacetate-coenzyme A ligase (paaK) from Azoarcus evansii

KEGG orthology group: K01912, phenylacetate-CoA ligase [EC: 6.2.1.30] (inferred from 100% identity to rpa:RPA1723)

MetaCyc: 65% identical to phenylacetate-CoA ligase (aerobic) (Aromatoleum evansii)
Phenylacetate--CoA ligase. [EC: 6.2.1.30]

Predicted SEED Role

"Phenylacetate-coenzyme A ligase (EC 6.2.1.30)" in subsystem Aromatic amino acid interconversions with aryl acids (EC 6.2.1.30)

MetaCyc Pathways

• phenylacetate degradation I (aerobic) (5/9 steps found)
• penicillin G and penicillin V biosynthesis (1/4 steps found)
• phenylacetate degradation II (anaerobic) (1/4 steps found)
• superpathway of phenylethylamine degradation (6/11 steps found)
• L-phenylalanine degradation IV (mammalian, via side chain) (4/9 steps found)
• anaerobic aromatic compound degradation (Thauera aromatica) (8/27 steps found)

KEGG Metabolic Maps

• Phenylalanine metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.2.1.30

Use Curated BLAST to search for 6.2.1.30

Protein Sequence (443 amino acids)

>TX73_008865 phenylacetate--CoA ligase (Rhodopseudomonas palustris CGA009)
MAATVHQIKPGTYNPELDDAERASRDEIVALQTKRLAWSLKHAYDNVAHYKAAFDRKGVH
PSDFRQLSDLSKFPFTVKTDLRDNYPFAMFAVPRESLVRVHASSGTTGKPIVVGYTPKDI
DTWATVMARSLRAAGARRGMIIHNSYGYGLFTGGLGAHYGGEKLGCTVVPVSGGMTERQV
QLINDFRPDIITVTPSYMLAILDEFKRQGLDPRQSSLKYGVFGAEPWTNAMRAEIEQAFD
MDATDIYGLSEVIGPGVAQECVETKDGLHIWEDHFYPEVIDPDTGDVLPDGEKGELVFTS
LTKEAFPVIRYRTRDLTRLLPGTARPGMRRMEKITGRSDDMIILRGVNVFPTQIEEVLLG
TDWCGGHFIIELTREGRMDEMTVLAEARPESWDGAGLVPHAERAAAYIKNTIGISTTIKV
VPPDTLERSLGKARRVFDKRPKG