Protein Info for TX73_008265 in Rhodopseudomonas palustris CGA009

Annotation: benzoylformate decarboxylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02776: TPP_enzyme_N
  • amino acids 15 to 117 (103 residues), 89.1 bits, E=2.8e-29
• PF00205: TPP_enzyme_M
  • amino acids 200 to 330 (131 residues), 58.6 bits, E=8.3e-20
• PF02775: TPP_enzyme_C
  • amino acids 389 to 528 (140 residues), 115.6 bits, E=2.7e-37

Best Hits

KEGG orthology group: K01576, benzoylformate decarboxylase [EC: 4.1.1.7] (inferred from 100% identity to rpa:RPA1609)

Predicted SEED Role

"Benzoylformate decarboxylase (EC 4.1.1.7)" in subsystem Benzoate degradation (EC 4.1.1.7)

MetaCyc Pathways

• D-phenylglycine degradation (1/3 steps found)
• 4-hydroxymandelate degradation (2/6 steps found)
• mandelate degradation I (1/5 steps found)
• indole-3-acetate degradation II (1/7 steps found)
• mandelate degradation to acetyl-CoA (6/18 steps found)
• superpathway of aromatic compound degradation via 3-oxoadipate (11/35 steps found)
• superpathway of aromatic compound degradation via 2-hydroxypentadienoate (9/42 steps found)

KEGG Metabolic Maps

• Benzoate degradation via hydroxylation
• Toluene and xylene degradation

Isozymes

Compare fitness of predicted isozymes for: 4.1.1.7

Use Curated BLAST to search for 4.1.1.7

Protein Sequence (542 amino acids)

>TX73_008265 benzoylformate decarboxylase (Rhodopseudomonas palustris CGA009)
MPAKKSKQPSAAVTTVKSATLDLLRAFKIDKVFGNPGSTELPFLSDWPDDIDYVLALQEA
SAVAMADGYAQATRNAGFVNLHSAAGVGNALGNIYSAFKNQTPLVITAGQQARSLLPLQA
FLGAERASEFPRPYVKYSVEPARAEDVPAAIARAYYVAMQPPCGPTFVSVPIDDWARPAA
PVPPRTITREIGPDRSAMQVLADTLANAKKPALVVGPAIDRAAAVGLMARLAERAKAPVW
VSPFSARCSFPERHPLFAGFLPASPGQLSETLGAYDVIVLIGAPVFTFHVEGHAAIFDGA
SQLFQITDDAEAASVTPLGASIIATMTPALTLLLELLPETKRAAPPARAVPPAPRPAEPM
PVEYLLHTLRAAMPESAMLVEEAPSHRPAMQTYMPMPGQDSFATMASGGLGWSLPASVGF
ALAHPNRRTVCLIGDGSAMYSIQALWTAAQRKLPLTVVVLNNGGYGAMRSFSQVMQVRNV
PGLELPGIDFTALAQSLGCDAVRVTRSEELAPALTRALAWDGVSLVEVMLDTSVPMLYAR
NG