Protein Info for TX73_007380 in Rhodopseudomonas palustris CGA009

Annotation: nitrogenase iron-iron protein, alpha chain

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 522 TIGR01861: nitrogenase iron-iron protein, alpha chain" amino acids 1 to 512 (512 residues), 1085.6 bits, see alignment E=0 TIGR01284: nitrogenase alpha chain" amino acids 4 to 459 (456 residues), 854.2 bits, see alignment E=2.7e-261 TIGR01862: nitrogenase component I, alpha chain" amino acids 14 to 454 (441 residues), 708.4 bits, see alignment E=4.7e-217 PF00148: Oxidored_nitro" amino acids 49 to 447 (399 residues), 282.1 bits, see alignment E=3.4e-88

Best Hits

Swiss-Prot: 85% identical to ANFD_AZOVI: Nitrogenase iron-iron protein alpha chain (anfD) from Azotobacter vinelandii

KEGG orthology group: K02586, nitrogenase molybdenum-iron protein alpha chain [EC: 1.18.6.1] (inferred from 100% identity to rpt:Rpal_1622)

MetaCyc: 85% identical to dinitrogenase 3 subunit alpha (Azotobacter vinelandii)

Predicted SEED Role

"Nitrogenase (iron-iron) alpha chain (EC 1.18.6.1)" in subsystem Nitrogen fixation (EC 1.18.6.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.18.6.1

Use Curated BLAST to search for 1.18.6.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (522 amino acids)

>TX73_007380 nitrogenase iron-iron protein, alpha chain (Rhodopseudomonas palustris CGA009)
MPYHEFDCSKCLPERQKHAVTKGAGDNLATALPLGYLNTIPGSISERGCAYCGAKHVIGT
PMKDVIHMSHGPVGCTYDTWQTKRYISDNNNFQLKYTFATDVREKHIVFGAEGLLKQNII
EAFKACPDIKRMTIYQTCATALIGDDINAVAAEVMEEMPDVDIFTCNSPGFGGPSQSGGH
HKINIAWINDKVGTVEPEITSDYVINYVGEYNIQGDQEVMLDYFTRMGIQVLSTFTGNGT
YDGLRAMHRAHLNVLECARSAEYICNELRVRYGIPRLDIDGFGFEPLSQSLRKIGMFFGI
EDRAEAIIAEETARWKPELDWYKERLKGKKVCLWPGGSKLWHWAHAIEEEMGVKVVSVYT
KFGHQGDMEKGIARCGEDALAIDDPNELEGLEALEKLQPDIIFTGKRPGEVAKKVRVPYL
NAHAYHNGPYKGFEGWVRFARDIYNGIYSPMHQLSGLDISKDEIPADRGFVTQRMLSDAK
LPEEIAKSETLRRYTGKDDIISDLRKKNAPYFTPIVKAEAAE