Protein Info for TX73_007335 in Rhodopseudomonas palustris CGA009

Annotation: phenylacetate--CoA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00501: AMP-binding
  • amino acids 88 to 298 (211 residues), 57.3 bits, E=1.2e-19
• PF14535: AMP-binding_C_2
  • amino acids 348 to 440 (93 residues), 76.9 bits, E=1.2e-25

Best Hits

KEGG orthology group: K01912, phenylacetate-CoA ligase [EC: 6.2.1.30] (inferred from 100% identity to rpa:RPA1429)

Predicted SEED Role

"Phenylacetate-coenzyme A ligase (EC 6.2.1.30)" in subsystem Aromatic amino acid interconversions with aryl acids (EC 6.2.1.30)

MetaCyc Pathways

• phenylacetate degradation I (aerobic) (5/9 steps found)
• penicillin G and penicillin V biosynthesis (1/4 steps found)
• phenylacetate degradation II (anaerobic) (1/4 steps found)
• superpathway of phenylethylamine degradation (6/11 steps found)
• L-phenylalanine degradation IV (mammalian, via side chain) (4/9 steps found)
• anaerobic aromatic compound degradation (Thauera aromatica) (8/27 steps found)

KEGG Metabolic Maps

• Phenylalanine metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.2.1.30

Use Curated BLAST to search for 6.2.1.30

Protein Sequence (445 amino acids)

>TX73_007335 phenylacetate--CoA ligase (Rhodopseudomonas palustris CGA009)
MTTTSDERPYWDAKLETQSRADWGALKLSLLQKHIAHAYAGSPAYRAAFDAAKVSPAQIK
SLDDIRRFPFIDKRTLRDRQLAVPPFGDLVAVPEQDIVYISASSGSTGVPTASPFTQSDF
DGWIDYEARQFWSSGMRPSDRYVHSLNFSLFIGGPCVLGAQKLGALTIHAGTIPSDRLLQ
ILRQFQATVIWTTPSYAWYLGETAIKEGYDLRKDLAVRRIFVAGEPGGSIPETRNRIEQL
WGASVYDYYGLSDIFGSCAGMCEQKEGLHWAEDHILVEVIDPDSGEPVKPGDRGEMVLTT
LKKTARPMIRFRTGDIVSFNPEPCRCGRTAIRLNGVHGRRDDMLIIKGVNLFPSDVEAVA
RQDHELTGEYRLVVDRINHLDRLTVEVEHIHGYNGAPETLADRFERKLKAVTGVSAAVSV
LKPDTLPRATHKAKRVEDRRSGVWS