Protein Info for TX73_007205 in Rhodopseudomonas palustris CGA009
Annotation: allophanate hydrolase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K02433, aspartyl-tRNA(Asn)/glutamyl-tRNA (Gln) amidotransferase subunit A [EC: 6.3.5.6 6.3.5.7] (inferred from 100% identity to rpa:RPA1404)Predicted SEED Role
"Allophanate hydrolase (EC 3.5.1.54)" in subsystem Urea decomposition (EC 3.5.1.54)
MetaCyc Pathways
- L-citrulline biosynthesis (7/8 steps found)
- L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
- L-citrulline degradation (3/3 steps found)
- ammonia assimilation cycle III (3/3 steps found)
- cyanate degradation (3/3 steps found)
- urea degradation I (3/3 steps found)
- L-glutamate biosynthesis I (2/2 steps found)
- cyanuric acid degradation II (4/5 steps found)
- superpathway of L-citrulline metabolism (9/12 steps found)
- L-glutamine degradation I (1/1 steps found)
- L-arginine degradation V (arginine deiminase pathway) (3/4 steps found)
- glutaminyl-tRNAgln biosynthesis via transamidation (3/4 steps found)
- L-glutamate and L-glutamine biosynthesis (5/7 steps found)
- superpathway of allantoin degradation in yeast (4/6 steps found)
- cyanuric acid degradation I (3/5 steps found)
- uracil degradation III (3/5 steps found)
- superpathway of atrazine degradation (4/8 steps found)
- allantoin degradation IV (anaerobic) (2/9 steps found)
KEGG Metabolic Maps
- Alanine and aspartate metabolism
- Aminoacyl-tRNA biosynthesis
- Arginine and proline metabolism
- Atrazine degradation
- Glutamate metabolism
- Urea cycle and metabolism of amino groups
Isozymes
Compare fitness of predicted isozymes for: 3.5.1.54, 6.3.5.6, 6.3.5.7
Use Curated BLAST to search for 3.5.1.54 or 6.3.5.6 or 6.3.5.7
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (607 amino acids)
>TX73_007205 allophanate hydrolase (Rhodopseudomonas palustris CGA009) MSAAELTTFPTIATLHTAYAAGASPAEIIAATYRRLEAVADPGIFITLRPEHDVLADAAA LGPFDPDTKPLWGIPFAVKDNIDVAGLPTTAACPGFASTPSETAFAVQRLLDAGAVLIGK TNLDQFATGLVGVRTPYPVPRNAIDPRYVPGGSSSGSAVVVAHGLVTFALGTDTAGSGRV PAALNNIVGLKPSLGSVSSRGMVPACRTLDTISVFAGTVADAHAVYGVIAAFDREDPWSR PHAGAPARLNALSPGLRIGLPNPASRKFADDLESEHAFDLAVADLATVVPGPVRAVDLTP LFDVASLLYSGPWVAERYQAIRHVIETTPELLHPVTRKIIGSASAFSAADAFGGLYRLAE LRHAADAIWSSVDVLMVPTYPRPRTIADLESDPIGPNSELGTYTNFVNLLDLCALAVPGR FRDDGFPSGVTLIAPAGHDDLLAVLGGRLHAASDVTLGASSTKVPAPVEASSTAAANEIE LVVVGAHLSGMPLNHELTSRGARFLRAIPTAPDYKLFALQGGPPFRPGLLRVAPGEGTPI ATEVWAISAEGFGSFVAGIPAPLGIGTTRLADGTAPKGFIVEAEGLKAATDISSFGGWRA YIRSRGG