Protein Info for TX73_005990 in Rhodopseudomonas palustris CGA009

Annotation: 50S ribosomal protein L3 N(5)-glutamine methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 340 TIGR03533: protein-(glutamine-N5) methyltransferase, ribosomal protein L3-specific" amino acids 49 to 336 (288 residues), 388 bits, see alignment E=2.6e-120 TIGR00536: methyltransferase, HemK family" amino acids 51 to 317 (267 residues), 209 bits, see alignment E=7.7e-66 PF05175: MTS" amino acids 161 to 254 (94 residues), 61.4 bits, see alignment E=2.6e-20 PF06325: PrmA" amino acids 175 to 249 (75 residues), 30.7 bits, see alignment E=7e-11 PF13847: Methyltransf_31" amino acids 176 to 306 (131 residues), 40.9 bits, see alignment E=5.6e-14 PF13649: Methyltransf_25" amino acids 178 to 249 (72 residues), 37.6 bits, see alignment E=9.1e-13

Best Hits

Swiss-Prot: 73% identical to PRMB_BRADU: 50S ribosomal protein L3 glutamine methyltransferase (prmB) from Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)

KEGG orthology group: K07320, putative adenine-specific DNA-methyltransferase [EC: 2.1.1.72] (inferred from 100% identity to rpa:RPA1167)

Predicted SEED Role

"Protein-N(5)-glutamine methyltransferase PrmB, methylates LSU ribosomal protein L3p"

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.72

Use Curated BLAST to search for 2.1.1.72

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (340 amino acids)

>TX73_005990 50S ribosomal protein L3 N(5)-glutamine methyltransferase (Rhodopseudomonas palustris CGA009)
MAKKTKAKAAKAARPKAAKTAVAKAKPAKRGVAARSVAAKPPKVAPGELVTLLDFVRYAV
SRFAEAGVVFAHGTTDPIAEAVFLVGEALHLHPDQFEMFASARVTTREAAAILGLIERRI
GSRLPAAYLVNKIYMRGVPFYVDERVIVPRSYIGELLDSHFDGGDTSLIDAPEAIERVLD
LCTGSGCLAILAAYAFPNATVDAVDLSKDALAVATRNVAEHRLDDRVSLYHGDLFGPLGD
ERYDLIITNPPYVDADGMASLPAECRAEPAMAFDGGDDGLTIIRRILAEAKHHLNPDGGL
LCEVGRCRPQIEDEFPQLPLLWLDSEDSEAEVFWIAAADL