Protein Info for TX73_005815 in Rhodopseudomonas palustris CGA009

Annotation: glycolate oxidase subunit GlcF

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 437 PF13183: Fer4_8" amino acids 22 to 90 (69 residues), 43.8 bits, see alignment E=1.2e-14 PF13237: Fer4_10" amino acids 23 to 87 (65 residues), 24.7 bits, see alignment E=7.9e-09 PF13534: Fer4_17" amino acids 24 to 91 (68 residues), 39.9 bits, see alignment E=2e-13 PF12838: Fer4_7" amino acids 24 to 90 (67 residues), 41.4 bits, see alignment E=6.4e-14 PF02754: CCG" amino acids 194 to 281 (88 residues), 43.5 bits, see alignment E=1.1e-14 amino acids 327 to 410 (84 residues), 49 bits, see alignment E=2.1e-16

Best Hits

KEGG orthology group: K11473, glycolate oxidase iron-sulfur subunit (inferred from 100% identity to rpa:RPA1132)

Predicted SEED Role

"Glycolate dehydrogenase (EC 1.1.99.14), iron-sulfur subunit GlcF" in subsystem Glycolate, glyoxylate interconversions or Photorespiration (oxidative C2 cycle) (EC 1.1.99.14)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.99.14

Use Curated BLAST to search for 1.1.99.14

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (437 amino acids)

>TX73_005815 glycolate oxidase subunit GlcF (Rhodopseudomonas palustris CGA009)
MKTDFSLTQLADPDIAEADKILRACVHCGFCTATCPTYVLLGDELDSPRGRIYLIKEMLE
SDKPPTADVVKHIDRCLSCLACMTTCPSGVNYMHLVDQARVRIEKQYQRPFWDRVVREAL
SWMMPHPGMFRLGMWAARIVRPVAALLPGSHNLTHPTLLSRIKAMLALAPKHLPEPGPDS
GTTFPAVGPKRGRVALLHGCAQQVLAPRINRAAINLLTRHGIEVVLAADEACCGALIHHL
GRDTRTLEYARTNIKAWLAEIERGGLDAILVTTSGCGTVIKDYGYMLREDPEYAAAAAKV
SALAKDISEYVSGLELSLPRPHDDVVVAYHSACSLQHGQKITQIPKELLSKTGFVVKDIP
ESHLCCGSAGTYNILQPDIATRLRDRKVANIASVKPDMIAAGNIGCMVQIASGTEVPVVH
TIELLDWATGGPRPAPH