Protein Info for TX73_005000 in Rhodopseudomonas palustris CGA009

Annotation: [NiFe]-hydrogenase assembly chaperone HybE

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 174 PF11939: NiFe-hyd_HybE" amino acids 17 to 167 (151 residues), 156.6 bits, see alignment E=2.3e-50 TIGR03993: [NiFe] hydrogenase assembly chaperone, HybE family" amino acids 20 to 166 (147 residues), 155.5 bits, see alignment E=4.2e-50

Best Hits

Swiss-Prot: 47% identical to HUPJ_BRADU: Hydrogenase expression/formation protein HupJ (hupJ) from Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)

KEGG orthology group: None (inferred from 100% identity to rpa:RPA0971)

Predicted SEED Role

"Hydrogenase maturation factor HoxT/HybE"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (174 amino acids)

>TX73_005000 [NiFe]-hydrogenase assembly chaperone HybE (Rhodopseudomonas palustris CGA009)
MTAEVAAHRDVEARAAGEAVAAIYRAAVPAMRDLPVFNPALDVAAIGFRALDDHAFGVIV
TPWFMNLVRLPLDPATAGRSQGEVVTRVLPVGALEFTAGQLDGIGGIESCSLFSPMFDFA
DQSAAEAAAEAALAAVLEPEQVADETSPSQAPSKMATTLDRRGLLRGAWMERRP