Protein Info for TX73_003465 in Rhodopseudomonas palustris CGA009
Annotation: bifunctional 4-hydroxybenzoate--CoA/benzoate--CoA ligase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 100% identical to 4HBCL_RHOPA: 4-hydroxybenzoate--CoA/benzoate--CoA ligase (hbaA) from Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009)
KEGG orthology group: K04105, 4-hydroxybenzoate-CoA ligase [EC: 6.2.1.27] (inferred from 100% identity to rpa:RPA0669)MetaCyc: 100% identical to 4-hydroxybenzoate-CoA ligase subunit (Rhodopseudomonas palustris CGA009)
4-hydroxybenzoate--CoA ligase. [EC: 6.2.1.27]; Benzoate--CoA ligase. [EC: 6.2.1.27, 6.2.1.25]
Predicted SEED Role
"Benzoate-CoA ligase (EC 6.2.1.25)" in subsystem Benzoate transport and degradation cluster (EC 6.2.1.25)
MetaCyc Pathways
- 4-coumarate degradation (anaerobic) (5/6 steps found)
- benzoate degradation II (aerobic and anaerobic) (1/1 steps found)
- phenol degradation II (anaerobic) (2/4 steps found)
- benzoate biosynthesis I (CoA-dependent, β-oxidative) (4/9 steps found)
- benzoate fermentation (to acetate and cyclohexane carboxylate) (7/17 steps found)
- anaerobic aromatic compound degradation (Thauera aromatica) (8/27 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 6.2.1.25
Use Curated BLAST to search for 6.2.1.25 or 6.2.1.27
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (539 amino acids)
>TX73_003465 bifunctional 4-hydroxybenzoate--CoA/benzoate--CoA ligase (Rhodopseudomonas palustris CGA009) MPLRDYNAAVDFVDRNVAEGRGGKIAFIDPQRSLSYGELRDAVARVGPMLARLGVEQENR IALVLKDTVDFPILFWGAIRAGIVPVLLNTRLTADQYRYLLEDSRSRVVFASSEFLPVIE EAAADLPHLRTIIAVGDAPAPTLQLANLLATEQEGGAPAATCADDIAYWQYSSGTTGMPK GVMHVHSSPRVMAENAGRRIGYREDDVVFSAAKLFFAYGLGNAMFCPMGIGATSVLYPER PTADSVFDTLRLHQPTLLFAVPTLYAAMLADPRSRTETLPDRLRLCVSAGEPLPAQVGLN WRNRFGHDIVNGVGSTEMGHLFLTNLPHAVEYGTSGVPVDGYRLRLVGDRGQDVADDEIG ELLVSGGSSAAGYWNQRDKTRTTFVGEWTRTGDKYHRRADGVYTYCGRTDDIFKVSGIWV SPFEIEQALMSHAKVLEAAVIPAEDTDGLIKPKAFIVLASRGDIDPGALFDELKEHVKSA IGPWKYPRWIQIMDDLPKTSSGKLQRYLLREMTLGGIEATESAPSEPALYGRVVAGNGR