Protein Info for TX73_003425 in Rhodopseudomonas palustris CGA009

Annotation: benzoate-CoA ligase family protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02262: benzoate-CoA ligase family
  • amino acids 11 to 521 (511 residues), 904.3 bits, E=1.1e-276
• PF00501: AMP-binding
  • amino acids 22 to 387 (366 residues), 280.5 bits, E=2e-87
• PF13193: AMP-binding_C
  • amino acids 437 to 512 (76 residues), 78.5 bits, E=6.1e-26

Best Hits

KEGG orthology group: K04110, benzoate-CoA ligase [EC: 6.2.1.25] (inferred from 100% identity to rpa:RPA0661)

Predicted SEED Role

"Benzoate-CoA ligase (EC 6.2.1.25)" in subsystem Benzoate transport and degradation cluster (EC 6.2.1.25)

MetaCyc Pathways

• benzoate degradation II (aerobic and anaerobic) (1/1 steps found)
• benzoate biosynthesis I (CoA-dependent, β-oxidative) (4/9 steps found)
• benzoate fermentation (to acetate and cyclohexane carboxylate) (7/17 steps found)
• anaerobic aromatic compound degradation (Thauera aromatica) (8/27 steps found)

KEGG Metabolic Maps

• Benzoate degradation via CoA ligation

Isozymes

Compare fitness of predicted isozymes for: 6.2.1.25

Use Curated BLAST to search for 6.2.1.25

Protein Sequence (524 amino acids)

>TX73_003425 benzoate-CoA ligase family protein (Rhodopseudomonas palustris CGA009)
MNAAAVTPPPEKFNFAEHLLRTNRVRPDKTAFVDDISSLSFAQLEAQTRQLAAALRAIGV
KREERVLLLMLDGTDWPVAFLGAIYAGIVPVAVNTLLTADDYAYMLEHSRAQAVLVSGAL
HPVLKAALTKSDHEVQRVIVSRPAAPLEPGEVDFAEFVGAQVPLEKPAATQADDPAFWLY
SSGSTGRPKGVVHTHANPYWTSELYGRNTLHLREDDVCFSAAKLFFAYGLGNALTFPMSV
GATTLLMGERPTPDAVFKRWLGGVGGVKPTVFYGAPTGYAGMLAAPNLPARDQVALRLAS
SAGEALPAEIGQRFQRHFGLDIVDGIGSTEMLHIFLSNLPDRVRYGTTGWPVPGYQIELR
GDGGGPVAAGEPGDLYIHGPSSATMYWGNRAKSRDTFQGGWTKSGDKYVRNDDGSYTYAG
RTDDMLKVSGIYVSPFEIEATLVQHPGVLEAAVVGVADEHGLTKPKAYVVPRPGQTLSET
ELKTFIKDRLAPYKYPRSTVFVAELPKTATGKIQRFKLREGVLG