Protein Info for TX73_003140 in Rhodopseudomonas palustris CGA009

Annotation: peptide chain release factor N(5)-glutamine methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 289 TIGR00536: methyltransferase, HemK family" amino acids 6 to 285 (280 residues), 204.3 bits, see alignment E=2.1e-64 PF17827: PrmC_N" amino acids 10 to 79 (70 residues), 66.4 bits, see alignment E=6.7e-22 TIGR03534: protein-(glutamine-N5) methyltransferase, release factor-specific" amino acids 29 to 282 (254 residues), 262.2 bits, see alignment E=4.9e-82 PF05175: MTS" amino acids 118 to 200 (83 residues), 47.3 bits, see alignment E=4.6e-16 PF13847: Methyltransf_31" amino acids 121 to 247 (127 residues), 37.3 bits, see alignment E=5.7e-13 PF13649: Methyltransf_25" amino acids 122 to 208 (87 residues), 35.5 bits, see alignment E=3.4e-12

Best Hits

Swiss-Prot: 61% identical to PRMC_BRADU: Release factor glutamine methyltransferase (prmC) from Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)

KEGG orthology group: K02493, methyltransferase [EC: 2.1.1.-] (inferred from 100% identity to rpa:RPA0607)

Predicted SEED Role

"Protein-N(5)-glutamine methyltransferase PrmC, methylates polypeptide chain release factors RF1 and RF2"

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.-

Use Curated BLAST to search for 2.1.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (289 amino acids)

>TX73_003140 peptide chain release factor N(5)-glutamine methyltransferase (Rhodopseudomonas palustris CGA009)
MSDAPTIASARRRLTRQFQDAGIESAALDARLLVGEVAGLDLTGLLIQAERILTAEENEQ
LQSLAARRLGGEPVARILGMREFWGLPFELSADTLVPRPDTETIVEAALAILAERSSLAP
RILDIGTGTGAILLALLHECPDAVGVATDISLGALRTARGNAQRLGLADRACFVACDYAS
ALCGPFDLIVSNPPYIPGNEIAALDREVRDHDPRRALDGGDDGLDAYRKIIPESVRLLQP
GGVLVVEIGQGQEGDVSALMQASGLTVSDPFRPDLSGIFRAVTGRLAVL