Protein Info for TX73_003065 in Rhodopseudomonas palustris CGA009

Annotation: bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00120: glutamate N-acetyltransferase/amino-acid acetyltransferase
  • amino acids 18 to 413 (396 residues), 380.1 bits, E=5.9e-118
• PF01960: ArgJ
  • amino acids 24 to 413 (390 residues), 470 bits, E=2.4e-145

Best Hits

Swiss-Prot: 100% identical to ARGJ_RHOPA: Arginine biosynthesis bifunctional protein ArgJ (argJ) from Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009)

KEGG orthology group: K00620, glutamate N-acetyltransferase / amino-acid N-acetyltransferase [EC: 2.3.1.1 2.3.1.35] (inferred from 100% identity to rpt:Rpal_0595)

Predicted SEED Role

"Glutamate N-acetyltransferase (EC 2.3.1.35) / N-acetylglutamate synthase (EC 2.3.1.1)" in subsystem Arginine Biosynthesis extended (EC 2.3.1.1, EC 2.3.1.35)

MetaCyc Pathways

• L-arginine biosynthesis II (acetyl cycle) (10/10 steps found)
• L-arginine biosynthesis I (via L-ornithine) (9/9 steps found)
• L-arginine biosynthesis III (via N-acetyl-L-citrulline) (8/9 steps found)
• L-ornithine biosynthesis I (5/5 steps found)
• superpathway of arginine and polyamine biosynthesis (13/17 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Urea cycle and metabolism of amino groups

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.1.1 or 2.3.1.35

Protein Sequence (413 amino acids)

>TX73_003065 bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ (Rhodopseudomonas palustris CGA009)
MSSKVSPLAPTDVPAMPMIAGVRLATAAAGIRYKGRTDVLLVEMEKGTTVAGVFTTSKCA
SAPVEWCRDKLKGGSARALVVNSGNANAFTGKSGKQAATLTAQLAAKAIGCKPAEVFLAS
TGVIGEPLDATKFDGVLDALALDAGPDGWMDAAKAIMTTDTFPKVATATVKLGKAKVTIN
GMAKGAGMIAPDMATMLSFIFTDAPLSASCLQALLKAGVTDTFNAVTIDGDTSTSDTLLA
FATGAAAENGAPKISRASDPRLKAFVKAFNAVLADLAEQVARDGEGARKLVEIIVEGAKT
KVSARKIALSIANSPLVKTAIAGEDANWGRVVMAVGKAGEPADRDKLSISFNGIRVAKAG
ARDPSYNEKEVSAEMKKPVIQIKVALGLGKGRDRVLTCDLTKEYVAINGDYRS