Protein Info for TX73_002690 in Rhodopseudomonas palustris CGA009

Annotation: flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 431 TIGR00612: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase" amino acids 18 to 407 (390 residues), 409.6 bits, see alignment E=6.1e-127 PF04551: GcpE" amino acids 21 to 407 (387 residues), 377.2 bits, see alignment E=3.6e-117

Best Hits

Swiss-Prot: 100% identical to ISPG_RHOPA: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin) (ispG) from Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009)

KEGG orthology group: K03526, (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase [EC: 1.17.7.1] (inferred from 100% identity to rpt:Rpal_0520)

Predicted SEED Role

"1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase (EC 1.17.7.1)" in subsystem Isoprenoid Biosynthesis (EC 1.17.7.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.17.7.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (431 amino acids)

>TX73_002690 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase (Rhodopseudomonas palustris CGA009)
MNKLENPLRDDVAGPAPRHQTTQVMVGDVAVGGGAPIVVQSMTNTDTADVEGTIKQIAAL
ARAGSEMVRITVDREEAAAAVPHIRDGIRKLGLTTPIIGDFHYIGHKLLAEYPACAEALD
KYRINPGNVGFKNKRDTQFADIVEIAIKNNKAVRIGANWGSLDQELLTKLMDENAASANP
RDVRAVTREAMVQSALLSAARAEEIGLPKNKMILSAKVSAVQDLIAVYQDLASRSDYAIH
LGLTEAGMGSKGIVASSAALGILLQQGIGDTIRISLTPEPGGDRTREVQVGQELLQTMGF
RTFVPLVAACPGCGRTTSTTFQELARSIQDFIRDEMPEWRSRYPGVENLNVAVMGCIVNG
PGESKHANIGISLPGTGETPAAPVFVDGEKFRTLRGENIAADFKALVIDYIEQRYGATPK
PDAAQMVPAAE