Protein Info for TX73_001975 in Rhodopseudomonas palustris CGA009

Annotation: FdhF/YdeP family oxidoreductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01701: oxidoreductase alpha (molybdopterin) subunit
  • amino acids 27 to 769 (743 residues), 911.9 bits, E=1.4e-278
• PF00384: Molybdopterin
  • amino acids 123 to 551 (429 residues), 62.6 bits, E=3.6e-21
• PF01568: Molydop_binding
  • amino acids 657 to 764 (108 residues), 31.3 bits, E=1.8e-11

Best Hits

Swiss-Prot: 49% identical to YDEP_ECO57: Protein YdeP (ydeP) from Escherichia coli O157:H7

KEGG orthology group: K00123, formate dehydrogenase, alpha subunit [EC: 1.2.1.2] (inferred from 100% identity to rpa:RPA0380)

Predicted SEED Role

"Putative formate dehydrogenase oxidoreductase protein" in subsystem Formate hydrogenase

MetaCyc Pathways

• formate oxidation to CO₂ (1/1 steps found)
• oxalate degradation VI (1/4 steps found)
• purine nucleobases degradation II (anaerobic) (15/24 steps found)
• oxalate degradation III (1/5 steps found)
• purine nucleobases degradation I (anaerobic) (6/15 steps found)
• superpathway of C1 compounds oxidation to CO₂ (3/12 steps found)

KEGG Metabolic Maps

• Glyoxylate and dicarboxylate metabolism
• Methane metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.2

Use Curated BLAST to search for 1.2.1.2

Protein Sequence (770 amino acids)

>TX73_001975 FdhF/YdeP family oxidoreductase (Rhodopseudomonas palustris CGA009)
MSQDEARIHDTDIPDTADGTVTYDGPAGGWGSLQGISQIFGREWSSPAAIETLMRQNKPK
GFMCVSCSWAKPADHHTFEFCENGAKATLWELTSARCTPEFFAEHSVTELRGWADYDLEM
QGRLTEPMRYDATTDHYVPCSWDEAFQSIGEHLRSLDPKSVIFYASGRASLETSYLYALF
ARLYGNNNLPDSSNMCHETTSVALKKLLGVGVGTVVFDDLAKCDAMFFFGQNTGSNSPRF
LHPLQEAAKRGVEIITFNPVREKGLESFVNPQNPAEMLTGRETRISSQYHQVKAGGDVAV
LIGLCKHVFARDDEARREGRRVLDVDFIEAHTHGLDEFEAKVRAISWDEIERESGLSREA
IESAGDVYVKAERVIGVYGMGLTQHAHGFLNVAMFVNLLLLRGNIGRDGTGISPVRGHSN
VQGQRTVGISEKPELVPLDKMAEQFGFDPPRDKGMNTVEACHGFLEGKVRAFISLGGNFV
RAIPERDAMEAAWTRMQLTVQIATKLNRSHLINGKAAYLLPCLGRTEQDIQATGPQAVTM
EDTFSCIQGSIGLRKPASEQLKSELAIVAGIAKATLPDNPKLKWDDWVGDYGLVRDLIAE
SYPEMFHDFNDRMFTPGGFYKGNAARERIWKTESGKAEFTTPDRLNSIGFDDAPGRYRLM
TLRSNDQFNTTIYGMSDRLRGIEGRRDVLLINPDEMARAGLSEGQEVWLEGDAGDGVHRE
AGPLRVTPFRLPDGCLGAYYPEMNSLMPVWHHDGPSKTPAAKSVPVRIRT