Protein Info for TX73_001305 in Rhodopseudomonas palustris CGA009

Annotation: tRNA (N(6)-L-threonylcarbamoyladenosine(37)-C(2))- methylthiotransferase MtaB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 423 TIGR00089: radical SAM methylthiotransferase, MiaB/RimO family" amino acids 3 to 418 (416 residues), 319.6 bits, see alignment E=3e-99 PF00919: UPF0004" amino acids 3 to 89 (87 residues), 62 bits, see alignment E=4.7e-21 TIGR01579: MiaB-like tRNA modifying enzyme" amino acids 6 to 408 (403 residues), 419.9 bits, see alignment E=1.4e-129 PF04055: Radical_SAM" amino acids 149 to 321 (173 residues), 81.5 bits, see alignment E=8.6e-27

Best Hits

KEGG orthology group: None (inferred from 100% identity to rpa:RPA0251)

Predicted SEED Role

"tRNA-t(6)A37 methylthiotransferase"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (423 amino acids)

>TX73_001305 tRNA (N(6)-L-threonylcarbamoyladenosine(37)-C(2))- methylthiotransferase MtaB (Rhodopseudomonas palustris CGA009)
MAVEVVTFGCRLNAFESELIRREAEGAGLADTIVVNSCAVTNEAVAQARQQIRKLKRARP
EARIIVTGCAAQTEPATFAAMAEVDRVIGNDDKTRSDAWHAAKGALEAGPSFGLDTEQKI
AVADIMAVREMAPHLLDGYQSGLPRVFVQVQNGCDHRCTFCIIPYGRGNSRSVPVGAVVE
QVRLLAERGHAEIVLTGVDLTSYGADLPGAPKLGTLVKKVLRHVPELQRLRISSIDQVEA
DRDLIDALATEPRLMPHLHLSLQAGDDLVLKRMKRRHCRADAVAFCEEVRLLRPDIALGA
DLIAGFPTETEEMFQRSLDLVEECGLTFLHVFPYSPRPGTPAARMPQLDGRVIRDRAARL
RAAGEAALQRRLDAEIGATRAVLIESPTQGRTEHFLPVAISGATPGDVQTLRIIGHDGAR
LTA