Protein Info for TX73_001045 in Rhodopseudomonas palustris CGA009

Annotation: aconitate hydratase AcnA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01341: aconitate hydratase 1
  • amino acids 19 to 903 (885 residues), 1363.7 bits, E=0
• PF00330: Aconitase
  • amino acids 77 to 569 (493 residues), 567.7 bits, E=2.2e-174
• PF00694: Aconitase_C
  • amino acids 699 to 831 (133 residues), 140.1 bits, E=5.7e-45

Best Hits

Swiss-Prot: 89% identical to ACNA_BRADU: Aconitate hydratase A (acnA) from Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)

KEGG orthology group: K01681, aconitate hydratase 1 [EC: 4.2.1.3] (inferred from 74% identity to azc:AZC_4461)

Predicted SEED Role

"Aconitate hydratase (EC 4.2.1.3) @ 2-methylisocitrate dehydratase (EC 4.2.1.99)" (EC 4.2.1.3, EC 4.2.1.99)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (23/26 steps found)
• superpathway of glyoxylate bypass and TCA (12/12 steps found)
• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (19/22 steps found)
• TCA cycle I (prokaryotic) (10/10 steps found)
• TCA cycle V (2-oxoglutarate synthase) (9/9 steps found)
• TCA cycle VII (acetate-producers) (9/9 steps found)
• partial TCA cycle (obligate autotrophs) (8/8 steps found)
• ethene biosynthesis V (engineered) (20/25 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (12/14 steps found)
• TCA cycle III (animals) (9/10 steps found)
• glyoxylate cycle (6/6 steps found)
• TCA cycle II (plants and fungi) (8/9 steps found)
• TCA cycle VI (Helicobacter) (8/9 steps found)
• nitrogen remobilization from senescing leaves (7/8 steps found)
• TCA cycle IV (2-oxoglutarate decarboxylase) (7/9 steps found)
• mixed acid fermentation (11/16 steps found)
• 2-methylcitrate cycle I (3/5 steps found)
• reductive TCA cycle I (7/11 steps found)
• 2-methylcitrate cycle II (3/6 steps found)
• reductive TCA cycle II (7/12 steps found)
• methylaspartate cycle (11/19 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Citrate cycle (TCA cycle)
• Glyoxylate and dicarboxylate metabolism
• Propanoate metabolism
• Reductive carboxylate cycle (CO2 fixation)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.2.1.3 or 4.2.1.99

Protein Sequence (905 amino acids)

>TX73_001045 aconitate hydratase AcnA (Rhodopseudomonas palustris CGA009)
MTSLDSFKCRKILKVGSKSYVYYSLPTAEKNGLKGISRLPYSMKVLLENLLRNEDDRTVK
KADIMAVAKWMRKKALEHEIAFRPARVLMQDFTGVPAVVDLAAMRNAMQKLGGDAEKINP
LVPVDLVIDHSVIVNFFGNNQAFKKNVAEEYKQNQERYEFLKWGQKAFSNFAVVPPGTGI
CHQVNLEYLAQTVWTKKEKMTIGRKTGTFEVAYPDTLVGTDSHTTMVNGLAVLGWGVGGI
EAEAAMLGQPLSMLLPEVVGFKLKGALKEGVTATDLVLTVTQMLRKQGVVGKFVEFFGPG
LDNLSVADKATIANMAPEYGATCGFFPVDGETIDYLKTSGRASARVALVEKYAKAQGLFR
TAKSPDPVFTVTLTLDLADVVPSLAGPKRPEGRVALPAVAEGFTTAMDAEYKKALDGARY
KVEGRNFDIGHGDVVIAAITSCTNTSNPSVLIGAGLLARNAAAKGLKAAPWVKTSLAPGS
QVVAEYLANSGLQKDLDKVGFNLVGFGCTTCIGNSGPLPEEISKSINDNGIVAAAVLSGN
RNFEGRVSPDVQANYLASPPLVVAYALAGSVTKNLAVDPIGTGKDGKPVYLKDIWPSTKE
INAFVKKYVTSKIFKARYADVFKGDTNWRKIKTVESETYKWNMSSTYVQNPPYFEGMTKQ
PEPITDVVDARVLALFGDKITTDHISPAGSIKLTSPAGKYLTERQVRPADFNQYGTRRGN
HEVMMRGTFANIRIKNHMLKGADGNIPEGGLTKHWPDGEQMSIYDAAMKYQEEKVPLVVF
AGAEYGNGSSRDWAAKGTRLLGVRAVICQSFERIHRSNLVGMGVLPLTFEEGTSWASLGL
KGDEKVTIRGLEGDLKPRQMLEAEITSAAGKRKRVPLLCRIDTLDELDYYRNGGILHYVL
RKLAA