Protein Info for TX73_000960 in Rhodopseudomonas palustris CGA009

Annotation: dihydrolipoyl dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01350: dihydrolipoyl dehydrogenase
  • amino acids 4 to 467 (464 residues), 562 bits, E=4.8e-173
• PF07992: Pyr_redox_2
  • amino acids 4 to 329 (326 residues), 245.4 bits, E=4.3e-76
• PF01134: GIDA
  • amino acids 5 to 149 (145 residues), 27.7 bits, E=7.6e-10
• PF12831: FAD_oxidored
  • amino acids 5 to 54 (50 residues), 32.7 bits, 2.8e-11
• PF03486: HI0933_like
  • amino acids 5 to 39 (35 residues), 24.2 bits, 6.8e-09
• PF00890: FAD_binding_2
  • amino acids 5 to 41 (37 residues), 30.7 bits, 9.7e-11
• PF13450: NAD_binding_8
  • amino acids 8 to 43 (36 residues), 28.9 bits, 5.9e-10
• PF00070: Pyr_redox
  • amino acids 176 to 253 (78 residues), 75.2 bits, E=2.5e-24
• PF02852: Pyr_redox_dim
  • amino acids 348 to 457 (110 residues), 144.9 bits, E=5.2e-46

Best Hits

Swiss-Prot: 59% identical to DLDH2_ARATH: Dihydrolipoyl dehydrogenase 2, mitochondrial (LPD2) from Arabidopsis thaliana

KEGG orthology group: K00382, dihydrolipoamide dehydrogenase [EC: 1.8.1.4] (inferred from 100% identity to rpa:RPA0185)

MetaCyc: 58% identical to glycine cleavage system L protein 1 (Arabidopsis thaliana col)
GCVMULTI-RXN [EC: 1.4.1.27]

Predicted SEED Role

"Dihydrolipoamide dehydrogenase of 2-oxoglutarate dehydrogenase (EC 1.8.1.4)" in subsystem TCA Cycle (EC 1.8.1.4)

MetaCyc Pathways

• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (19/22 steps found)
• folate transformations III (E. coli) (8/9 steps found)
• photorespiration I (8/9 steps found)
• photorespiration III (8/9 steps found)
• 2-oxoglutarate decarboxylation to succinyl-CoA (3/3 steps found)
• glycine biosynthesis II (3/3 steps found)
• glycine cleavage (3/3 steps found)
• pyruvate decarboxylation to acetyl CoA I (3/3 steps found)
• photorespiration II (8/10 steps found)
• folate transformations II (plants) (8/11 steps found)
• glycine degradation (2/3 steps found)
• 2-oxoisovalerate decarboxylation to isobutanoyl-CoA (1/3 steps found)
• folate transformations I (8/13 steps found)
• reductive glycine pathway of autotrophic CO₂ fixation (5/9 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Citrate cycle (TCA cycle)
• Glycine, serine and threonine metabolism
• Glycolysis / Gluconeogenesis
• Pyruvate metabolism
• Valine, leucine and isoleucine degradation

Isozymes

Compare fitness of predicted isozymes for: 1.8.1.4

Use Curated BLAST to search for 1.4.1.27 or 1.8.1.4

Protein Sequence (467 amino acids)

>TX73_000960 dihydrolipoyl dehydrogenase (Rhodopseudomonas palustris CGA009)
MATYDLVVIGTGPGGYVCAIRAAQLGLKVAVVEKNATLGGTCLNVGCMPSKALLHASELF
EEAGHSFAKMGIGVSAPKLDLPAMMNFKQQGIDGNVKGVEYLMKKNKIDVLVGKGKILGT
GKVEVTGADGKATSVEAKSIVIASGSAVAQLKGIEIDEKRVVSSTGALSLDKVPGKLIVV
GAGVIGLELGSVWRRLGAEVTVVEFLDRILPGMDGEVVKQFQRILEKQGFAFKLGAKVTG
VDTSGAKLAVKVEAAAGGNPETLEADVVLVAIGRVPYTEGLGLKEAGVALDERGRVVIDD
HFATSLKGVYAIGDVVRGPMLAHKAEDEGVAVAELIAGKAGHVNYDVIPGVVYTTPEVSS
VGKTEEDLKQAGVAYTVGKFPFTANGRSKVNQTTDGFVKILADAKTDRVLGVHIIGREAG
EMIHEAAVLMEFGGSAEDLARTCHAHPTRSEAIKEAALTVGKRAIHM