Protein Info for TX73_000695 in Rhodopseudomonas palustris CGA009

Annotation: FAD-dependent oxidoreductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 673 PF10589: NADH_4Fe-4S" amino acids 72 to 153 (82 residues), 70.8 bits, see alignment E=2.5e-23 PF14691: Fer4_20" amino acids 170 to 247 (78 residues), 62.8 bits, see alignment E=8.9e-21 PF07992: Pyr_redox_2" amino acids 268 to 571 (304 residues), 102.4 bits, see alignment E=1.1e-32 PF01266: DAO" amino acids 269 to 304 (36 residues), 28.7 bits, see alignment 3.4e-10 PF13450: NAD_binding_8" amino acids 272 to 307 (36 residues), 33.8 bits, see alignment 1.2e-11 PF00070: Pyr_redox" amino acids 407 to 481 (75 residues), 28 bits, see alignment E=9.2e-10

Best Hits

KEGG orthology group: K15022, formate dehydrogenase beta subunit [EC: 1.2.1.43] (inferred from 100% identity to rpa:RPA0135)

Predicted SEED Role

"Formate dehydrogenase-O, major subunit (EC 1.2.1.2)" in subsystem Formate hydrogenase (EC 1.2.1.2)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.2

Use Curated BLAST to search for 1.2.1.2 or 1.2.1.43

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (673 amino acids)

>TX73_000695 FAD-dependent oxidoreductase (Rhodopseudomonas palustris CGA009)
MRAVPWGGVEMTQVVYGVWDGVAYDSRAAEARAADYALANFDEFDEGNAIRAFIADRGFF
VFDPTVSLVDALFHYLKAAAEQSCGACTPCRIGTVLVRDALDQMRRGLDAALTLDDIVML
GEQIRQTSLCGLGQTCAVALLAVLRDFRERIEQELAQHRPIPAQHGMAYVTAPCIEACPS
KVNVPRYIDYIRDGKPENSLGVLLQKYPMAATCGRVCVRYCEQACRRKFVDEAVGIKTLK
RYVADQQSGPHALKFTRDMIRKPLADGMRVAVVGAGPAGISCAYHLLLRGYHVDVFDKAG
QAGGMAQIGIPSYRLPKDTLALETDIIVDLGGRFLFDQRLGRDFSVDDLFARGYRAVFLG
LGCQQGARLGVDGEDAAHGGYFSGIDFLLKVHDHVDGIAPLVLQGEVVVVGGGNVAMDCV
RSAIRLGAEKVHVVYRRTLADMPADPAEIEAARAEGVEFHVLTAPAEIVTDHGKVTGVVL
TRMQASEPDAGGRRSVKPVPGSETSMRCDVLIAAIGQQVEDGPLIESDGIAFDRWRCVAT
DRVLATSRPGVFAGGDCVTGPSTLVYAMAAGLKAARNIDDWIQRGSVRFFKRSRMRKLIA
DNHMLANEIVAAPVRNAYRVHNPEIDPELRKHMFGEVEQTIDARAAYAETQRCMRCYRVY
SVVTKHPIPEGAA