Protein Info for TX73_000375 in Rhodopseudomonas palustris CGA009

Annotation: bifunctional folylpolyglutamate synthase/dihydrofolate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01499: bifunctional protein FolC
  • amino acids 30 to 436 (407 residues), 373.6 bits, E=7.8e-116
• PF08245: Mur_ligase_M
  • amino acids 53 to 269 (217 residues), 35.2 bits, E=6.4e-13

Best Hits

KEGG orthology group: K11754, dihydrofolate synthase / folylpolyglutamate synthase [EC: 6.3.2.12 6.3.2.17] (inferred from 100% identity to rpa:RPA0072)

Predicted SEED Role

"Dihydrofolate synthase (EC 6.3.2.12) @ Folylpolyglutamate synthase (EC 6.3.2.17)" (EC 6.3.2.12, EC 6.3.2.17)

MetaCyc Pathways

• tetrahydrofolate biosynthesis I (3/3 steps found)
• folate polyglutamylation (4/5 steps found)
• superpathway of tetrahydrofolate biosynthesis and salvage (9/12 steps found)
• tetrahydrofolate biosynthesis II (2/3 steps found)
• superpathway of tetrahydrofolate biosynthesis (7/10 steps found)
• superpathway of chorismate metabolism (40/59 steps found)

KEGG Metabolic Maps

• Biosynthesis of phenylpropanoids
• Folate biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.12 or 6.3.2.17

Protein Sequence (442 amino acids)

>TX73_000375 bifunctional folylpolyglutamate synthase/dihydrofolate synthase (Rhodopseudomonas palustris CGA009)
MSEGSAPPAPLGELRSRLTQLHPVAIDLTLGRIERLLAALGHPERKLPPVIHVAGTNGKG
STVAFLRAILEATELKVHAYTSPHLVRVNETIRIAGRLVGDDQLLTALAHCERVNAGAPI
TLFEIETAAALWLFAQHPADVTLLEVGLGGRLDATNVIEHPLACVLTPIGIDHTEFLGPT
IADIAAEKAGILRRGAPVIVAEQRDEAMEVIERDAKRLRAPLFACGQQWHVQLEHGRLAY
QDERGLLDLTAPKLFGRHQIDNAGLAIATLRAQSRFTIPQDAYQAGLLAAEWPARMQRIT
SGKLLDDAPAGCELWLDGGHNADGGRVAAAALGDLEERVSRPLVLIAGMMANKDAAAFLA
NFTGLTRHLIAVSIPNRDGAMPPERLADAGRALGLRVELADDVRAALRRIASLAYELPPR
ILITGSLYLAGHVLSLNGTPPN