Protein Info for Synpcc7942_2491 in Synechococcus elongatus PCC 7942

Name: gyrB
Annotation: DNA gyrase subunit B

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 645 TIGR01059: DNA gyrase, B subunit" amino acids 5 to 645 (641 residues), 1100 bits, see alignment E=0 PF02518: HATPase_c" amino acids 32 to 173 (142 residues), 62.7 bits, see alignment E=8.8e-21 PF00204: DNA_gyraseB" amino acids 228 to 398 (171 residues), 200.9 bits, see alignment E=2.2e-63 PF01751: Toprim" amino acids 427 to 538 (112 residues), 66.8 bits, see alignment E=3.3e-22 PF00986: DNA_gyraseB_C" amino acids 571 to 632 (62 residues), 108 bits, see alignment E=4.3e-35

Best Hits

Swiss-Prot: 63% identical to GYRB_ORYSJ: DNA gyrase subunit B, chloroplastic/mitochondrial (GYRB) from Oryza sativa subsp. japonica

KEGG orthology group: K02470, DNA gyrase subunit B [EC: 5.99.1.3] (inferred from 100% identity to syc:syc1615_c)

Predicted SEED Role

"DNA gyrase subunit B (EC 5.99.1.3)" in subsystem DNA topoisomerases, Type II, ATP-dependent or Resistance to fluoroquinolones (EC 5.99.1.3)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.3

Use Curated BLAST to search for 5.99.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8GIT7 at UniProt or InterPro

Protein Sequence (645 amino acids)

>Synpcc7942_2491 DNA gyrase subunit B (Synechococcus elongatus PCC 7942)
MANEYGASQIQVLEGLEPVRKRPGMYIGSTGPKGLHHLVYEVVDNAVDEALAGYCNTIDV
RLLEDGSCQVTDNGRGIPTDIHPQTGKSALETVLTILHAGGKFGGGGYKVSGGLHGVGVS
VVNALSEYVEVTVWREGKTHQQRFEQGNPIGELQVAPDADDRRGTQVRFKPDATIFSETT
EFDYGTLASRLKELAYLNAGVRIDFTDERLQLTKNHEPHQETYYFEGGIREYVAYMNTDK
QALHSEIIFVQSEKDGVQVEAALQWCVDAYSDNILGFANNIRTIDGGTHIEGLKTVLTRT
MNTIARKRNKRKDADNNLSGENIREGLTAIVSVKVPDPEFEGQTKTKLGNTEVRGIVDTL
VGETLTEYLEFHPSVADLILEKAIQAFNAAEAARRARELVRRKSVLESSTLPGKLADCSS
RDPGESEIFIVEGDSAGGSAKQGRDRRFQAILPLRGKILNIEKTDDAKIYKNTEIQALIT
ALGLGIKGEEFDASQLRYHRIVIMTDADVDGAHIRTLLLTFFYRYQRSLLEQGYIYIACP
PLYKLERGRNHYYCYNERELQERIATFPENANYTIQRFKGLGEMMPQQLWETTMDPETRT
MKRVEIEDAAEADRIFTILMGDRVQPRREFIETYGPQLSMAALDI