Protein Info for Synpcc7942_2490 in Synechococcus elongatus PCC 7942

Name: miaA
Annotation: tRNA delta(2)-isopentenylpyrophosphate transferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 306 signal peptide" amino acids 1 to 27 (27 residues), see Phobius details PF01745: IPT" amino acids 9 to 104 (96 residues), 32 bits, see alignment E=8.8e-12 TIGR00174: tRNA dimethylallyltransferase" amino acids 9 to 285 (277 residues), 304.1 bits, see alignment E=4.9e-95 PF01715: IPPT" amino acids 43 to 275 (233 residues), 260.3 bits, see alignment E=2e-81

Best Hits

Swiss-Prot: 100% identical to MIAA_SYNE7: tRNA dimethylallyltransferase (miaA) from Synechococcus elongatus (strain PCC 7942)

KEGG orthology group: K00791, tRNA dimethylallyltransferase [EC: 2.5.1.75] (inferred from 100% identity to syc:syc1616_d)

Predicted SEED Role

"tRNA dimethylallyltransferase (EC 2.5.1.75)" (EC 2.5.1.75)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.5.1.75

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8GIT6 at UniProt or InterPro

Protein Sequence (306 amino acids)

>Synpcc7942_2490 tRNA delta(2)-isopentenylpyrophosphate transferase (Synechococcus elongatus PCC 7942)
MESRLKPGLIVLCGPTAAGKSSLAIAIAQRLGSPILSADSRLVYRDFNIGTAKPTPAEQQ
QVPHYLMDLCDPRQVFTVGDYQDCAVPLIQQLQEKGMLPLLVGGTGLYIKAIVNGLRFPR
IAPQPKLRSQLQALGQPLCHALLQRVDPVAGDRIHVNDRVRTLRALEVFYVSGDRLTDLQ
QEQPPSYPILQIGLDSDRLEARIQQRTQQMLTSGFVEEVQGLCDRYGSDLPLLNTLGYRQ
VCAFLQGSLSRSELPEQIVLQTRQYAKQQRTWFRADSSIQWIDAEASNRLERALDLIERF
RKSEGV