Protein Info for Synpcc7942_2437 in Synechococcus elongatus PCC 7942

Name: ileS
Annotation: isoleucyl-tRNA synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 954 TIGR00392: isoleucine--tRNA ligase" amino acids 18 to 853 (836 residues), 1010.4 bits, see alignment E=3.6e-308 PF00133: tRNA-synt_1" amino acids 31 to 650 (620 residues), 460.6 bits, see alignment E=9.1e-142 PF09334: tRNA-synt_1g" amino acids 60 to 203 (144 residues), 43 bits, see alignment E=5.4e-15 PF08264: Anticodon_1" amino acids 697 to 851 (155 residues), 104.1 bits, see alignment E=1.3e-33 PF06827: zf-FPG_IleRS" amino acids 920 to 948 (29 residues), 44.8 bits, see alignment (E = 1.7e-15)

Best Hits

Swiss-Prot: 72% identical to SYI_NOSS1: Isoleucine--tRNA ligase (ileS) from Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576)

KEGG orthology group: K01870, isoleucyl-tRNA synthetase [EC: 6.1.1.5] (inferred from 72% identity to ana:alr1073)

Predicted SEED Role

"Isoleucyl-tRNA synthetase (EC 6.1.1.5)" (EC 6.1.1.5)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q31KF2 at UniProt or InterPro

Protein Sequence (954 amino acids)

>Synpcc7942_2437 isoleucyl-tRNA synthetase (Synechococcus elongatus PCC 7942)
VAEVSAYKDTLNLLQTPFNMRANAPVREPEIQQFWADRQIYETLSRQNPGAPFVLHDGPP
YANGALHMGHALNKTLKDIINKYQLLQGRKVRYVPGWDCHGLPIELKVLQELSSEERRNL
TPLTLRQKAKAYALAQVEQQSQSFQRYGVWADWDAPYLTLTPEYEAAQIDVFGQMVLKGY
IYRGLKPVHWSPSSRTALAEAELEYPDGHTSRSIYVAMPIVQLSEAAQPLLGNYANLALA
IWTTTPWTIPANLAVAVNGELTYAVVQAGDCHLIVAAELAESLSKTFATELTVLATFPGS
VLEHSRYRHPLYDRESPVVIGGDYITTESGTGLVHTAPGHGQDDFIVGNRYGLEVFCPVD
DKGDFTAAVGDRLVGKNVLKDANAAVIEWLTEVGALLKEESYAHRYPYDWRTKKPTIFRA
TEQWFASVEGFREQALQAIAEVDWIPAQGENRITSMVSERSDWCISRQRTWGVPIPVFYD
EESGEALLNAETIAHVRAIVAERGSDAWWELDVADLLPEPYRSNGRRYRKGTDTMDVWFD
SGSSWAAVASQREGLHYPADLYLEGSDQHRGWFQSSLLTSVACNGHAPYRRVLTHGFALD
EKGRKMSKSLGNVVDPAIVINGGKDQKQEPPYGADVLRLWVSSVDYSSDVPIGKNILKQM
ADVYRKIRNTARFLLGNLHDFDPAKDALPWEKLPELDRYLLHRLREVILEIQDAFESFQF
FRFFQTVQNFCVVDLSNFYLDIGKDRLYISAPDSLRRRSCQTVLAICVEALATAIAPVLS
HMAEDIWQSLPYPARTKSVFQAGWVQLQDDWNQPELAAKWQQLRDLRSEVNKVLEQARRD
QAIGSSLEAKLQLWVADSDWRAALADRNPADSLSGTAVDDLRYLFLVSQVELRDQPTGLT
EAKYHAQTEDWAIAVVDAEGQKCDRCWNYSTTVGQSSEHPDLCDRCVSALQGTF