Protein Info for Synpcc7942_2263 in Synechococcus elongatus PCC 7942

Name: hisS
Annotation: histidyl-tRNA synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 435 TIGR00442: histidine--tRNA ligase" amino acids 5 to 406 (402 residues), 501.8 bits, see alignment E=7.5e-155 PF13393: tRNA-synt_His" amino acids 9 to 311 (303 residues), 156.5 bits, see alignment E=1.5e-49 PF00587: tRNA-synt_2b" amino acids 67 to 316 (250 residues), 67.4 bits, see alignment E=2.6e-22 PF03129: HGTP_anticodon" amino acids 337 to 411 (75 residues), 61 bits, see alignment E=1.5e-20

Best Hits

Swiss-Prot: 100% identical to SYH_SYNE7: Histidine--tRNA ligase (hisS) from Synechococcus elongatus (strain PCC 7942)

KEGG orthology group: K01892, histidyl-tRNA synthetase [EC: 6.1.1.21] (inferred from 100% identity to syf:Synpcc7942_2263)

MetaCyc: 52% identical to histidine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Histidine--tRNA ligase. [EC: 6.1.1.21]

Predicted SEED Role

"Histidyl-tRNA synthetase (EC 6.1.1.21)" (EC 6.1.1.21)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.21

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q31KX6 at UniProt or InterPro

Protein Sequence (435 amino acids)

>Synpcc7942_2263 histidyl-tRNA synthetase (Synechococcus elongatus PCC 7942)
MASLQALRGTRDILPPETQVWQWIEQTAREILGRAAVQEVRTPIFEQTALFERGIGEATD
VVGKEMYSFRDRGDRSLTLRPEGTAGTVRAYIEHGLASQGGVQRLWYTGPMFRYERPQAG
RQRQFHQLGLELLGTADARADAEAIALATQILQALGLKNLRLDLNSVGDASDRAAYRQAL
VDYLTPYAADLDPDSRDRLERNPLRILDSKDERTQAIVAEAPSLHDYLSERSRQLFEQVQ
QLLTHLGIDYRLEPKLVRGLDYYTHTAFEIISSDLGAQATVCGGGRYDGLVSQLGGPETP
AVGWAMGLERLVLLLQQGQAVPPATLDFYLVSRGAIAEGQALILAQKLRSAGFGVELDLS
GSAFGKQFKRADRSGAIACLVLGDAEAEQGQVNLKWLQSGEQQTLDQSELLQDSDHWRSR
LQAARTVSPVEVAPL