Protein Info for Synpcc7942_2168 in Synechococcus elongatus PCC 7942

Name: pcrA
Annotation: ATP-dependent DNA helicase, Rep family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 794 TIGR01073: ATP-dependent DNA helicase PcrA" amino acids 5 to 791 (787 residues), 955 bits, see alignment E=1.4e-291 PF00580: UvrD-helicase" amino acids 9 to 318 (310 residues), 303.9 bits, see alignment E=4e-94 PF13245: AAA_19" amino acids 13 to 304 (292 residues), 79.8 bits, see alignment E=6.5e-26 PF13361: UvrD_C" amino acids 331 to 667 (337 residues), 266.2 bits, see alignment E=1.7e-82 PF13538: UvrD_C_2" amino acids 607 to 664 (58 residues), 31.9 bits, see alignment 2.8e-11 PF21196: PcrA_UvrD_tudor" amino acids 744 to 791 (48 residues), 32 bits, see alignment 2.8e-11

Best Hits

KEGG orthology group: K03657, DNA helicase II / ATP-dependent DNA helicase PcrA [EC: 3.6.4.12] (inferred from 100% identity to syf:Synpcc7942_2168)

Predicted SEED Role

"ATP-dependent DNA helicase UvrD/PcrA" in subsystem DNA repair, bacterial UvrD and related helicases

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.12

Use Curated BLAST to search for 3.6.4.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q31L71 at UniProt or InterPro

Protein Sequence (794 amino acids)

>Synpcc7942_2168 ATP-dependent DNA helicase, Rep family (Synechococcus elongatus PCC 7942)
MSSDFLSQLNTSQRRAVEHYSGPLLVVAGAGSGKTRALTFRIAHLLRQHRVDPENILAVT
FTNKAAKEMKERLERLFAQQLAELQYGQPFTTLREIEKTQLRSRVYREVTKDLWIGTFHA
ICARILRFDIDKYCDPNGRRWTKQFSIFDENDVQGLIKEIVGNQLNLDTKKFEPKSVRYT
ISSAKNNGWSPDEFERQQPNYRGRVIGEVYRRYLDQLAANNALDFDDLLLIPVQLFRQNE
QVLAYWHQRFRHVLVDEYQDTNRTQYDLIRLLTTNGAETRDFDQWNHRSVFVVGDADQSI
YSFRAADFTILMGFQDDFGDRLPDDDTRTMVKLEENYRSTENILQAANELIRNNTERIDK
VLRPTRGSGEAIACYRADDEMAEAEYVVQQIRQLEQQHPELNWGDFAILYRTNAQSRAFE
DSLVRWNVPYTVIGGLRFYDRREVKDILAYLRAIANPFDAVSLLRVVNVPKRGVGKTTIG
RLVDAAQELNVPLWEILSDSETVQNLAGRSAKGILEFANLVQHFQDQADELRPSELIRQL
LEASGYIAELQRSGTDEDEDRRRNLEELVNAAVQHEEENDDPSLIGFLGTASLASDADNK
EANQQVSLMTLHASKGLEFPVVFLVGLEQGLFPSFRSLDDPRAVEEERRLCYVGITRAQE
RLFLTHASARRLWGGGREPAIPSNFLSELPAELIVGHSPQTRRQTQANFKPPVTHERRAS
LRDRVAANAAKVQQVQQPARHWQVGDRLQHRQFGVGRITRIFGAGDRQSIVVQFSGATGQ
RILDPRLAPIEPLG