Protein Info for Synpcc7942_2091 in Synechococcus elongatus PCC 7942
Name: ndhF3
Annotation: NAD(P)H-quinone oxidoreductase subunit F
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K05577, NADH dehydrogenase I subunit 5 [EC: 1.6.5.3] (inferred from 100% identity to syf:Synpcc7942_2091)MetaCyc: 100% identical to ferredoxin-plastoquinone oxidoreductase subunit F3 (Synechococcus elongatus PCC 7942 = FACHB-805)
Carbonate dehydratase. [EC: 4.2.1.1]
Predicted SEED Role
"NADH dehydrogenase subunit 5, Involved in CO2 fixation" in subsystem CO2 uptake, carboxysome
MetaCyc Pathways
- cyanate degradation (3/3 steps found)
- CO2 fixation into oxaloacetate (anaplerotic) (2/2 steps found)
- C4 photosynthetic carbon assimilation cycle, NAD-ME type (7/11 steps found)
- C4 photosynthetic carbon assimilation cycle, NADP-ME type (4/7 steps found)
- C4 photosynthetic carbon assimilation cycle, PEPCK type (7/14 steps found)
- gluconeogenesis II (Methanobacterium thermoautotrophicum) (9/18 steps found)
- 3-hydroxypropanoate cycle (4/13 steps found)
- glyoxylate assimilation (3/13 steps found)
- 3-hydroxypropanoate/4-hydroxybutanate cycle (4/18 steps found)
- superpathway of the 3-hydroxypropanoate cycle (4/18 steps found)
- Methanobacterium thermoautotrophicum biosynthetic metabolism (19/56 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.6.5.3, 4.2.1.1
Use Curated BLAST to search for 1.6.5.3 or 4.2.1.1
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See Q8VPV9 at UniProt or InterPro
Protein Sequence (612 amino acids)
>Synpcc7942_2091 NAD(P)H-quinone oxidoreductase subunit F (Synechococcus elongatus PCC 7942) MLSAASQSVWLIPGYSLFGAIASLLWSPGLIRLTGPRPSGYVNVIASGVAFLHSLLALWA MQPAQGSFRWLNTVGFELSFDWSSTTAALVALTVITGLTWFSQIYAIGYLERDWGWARFF GYLSLFEAGLSGLVLCDSLFFSYVILEMLTLGTYLIVGYWYNQPLVLTGARDAFLTKRIG DLILLVGVLGLLPLAGTWNFDQLAEWASTAEPSLALTAIAIALIAGPLGKCAQFPFHLWL DEAMEGPLPSTILRNAIVVVSGAWVLIRIQPIIAAVPVAETLLISVGVVTAICASLIAIA QVDVKRALSYLVSTWMGLVFIAVGAGQTIAAERLLLVYSLSMALLMMSVGTIVIRNISQD ITQLGGLWSRRPLPALAFALGGLSLLAMPPFGGFWAWLGLAEGLWPVSPWLVIVLVFVLA AVGFGLARIFARIWGGPSKAMSVRSAEVLWLMVLPMLVLAGLTLHLPILLAQAGLLPQLN NLAIPLVWASLVGVATGTVVYYGPARNQPVQLPWQGLQNFFAQDFYTPILYRRTIVASVS FVATVLQFLDRTIVDGTATLMGLITLGSGQGLRYNVSGRTQGYALTMLLAIAVGSVFFLM PFLRQSFVLGLL