Protein Info for Synpcc7942_0713 in Synechococcus elongatus PCC 7942

Name: ispG
Annotation: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 407 TIGR00612: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase" amino acids 20 to 395 (376 residues), 514 bits, see alignment E=1.1e-158 PF04551: GcpE" amino acids 23 to 395 (373 residues), 423.1 bits, see alignment E=4e-131

Best Hits

Swiss-Prot: 100% identical to ISPG_SYNE7: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ferredoxin) (ispG) from Synechococcus elongatus (strain PCC 7942)

KEGG orthology group: K03526, (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase [EC: 1.17.7.1] (inferred from 100% identity to syf:Synpcc7942_0713)

Predicted SEED Role

"1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase (EC 1.17.7.1)" in subsystem Isoprenoid Biosynthesis (EC 1.17.7.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.17.7.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q31QC4 at UniProt or InterPro

Protein Sequence (407 amino acids)

>Synpcc7942_0713 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (Synechococcus elongatus PCC 7942)
MQTLSTPSTTATEFDTVIHRRPTRSVRVGDIWIGSRHPVVVQSMINEDTLDIDGSVAAIR
RLHEIGCEIVRVTVPSLGHAKAVGDIKKKLQDTYRDVPLVADVHHNGMKIALEVAKHVDK
VRINPGLYVFEKPDPNRQGYTPEEFERIGKQIRDTLEPLVTSLREQDKAMRIGVNHGSLA
ERMLFTYGDTPEGMVESALEFLRLCEEMDFRNLVISMKASRAPVMMAAYRLMAKRMDDLG
MDYPLHLGVTEAGDGDYGRIKSTVGIGTLLAEGIGDTIRVSLTEAPENEIPVCYSILQAL
GLRKTMVEYVACPSCGRTLFNLEEVLHKVRAATNHLVGLDIAVMGCIVNGPGEMADADYG
YVGKTPGTIALYRGRDEIKRVPEEQGVEELINLIKADGRWVEPEPIA