Protein Info for Synpcc7942_0056 in Synechococcus elongatus PCC 7942

Name: rfbE
Annotation: perosamine synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 39% identical to GDPPS_VIBCL: GDP-perosamine synthase (rfbE) from Vibrio cholerae

KEGG orthology group: None (inferred from 100% identity to syc:syc1444_d)

MetaCyc: 39% identical to GDP-4-dehydro-6-deoxy-D-mannose-4-aminotransferase subunit (Vibrio cholerae O1)
RXN-8953 [EC: 2.6.1.102]

Predicted SEED Role

"perosamine synthetase"

MetaCyc Pathways

GDP-D-perosamine biosynthesis (2/2 steps found)
superpathway of GDP-mannose-derived O-antigen building blocks biosynthesis (10/14 steps found)
GDP-N-acetyl-α-D-perosamine biosynthesis (2/3 steps found)
GDP-N-formyl-α-D-perosamine biosynthesis (2/3 steps found)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.6.1.102

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q31S81 at UniProt or InterPro

Protein Sequence (375 amino acids)

>Synpcc7942_0056 perosamine synthetase (Synechococcus elongatus PCC 7942)
MAKQSYRKIPYTKPSITELEIQYATDAARNGWGEHCYDYIIRFEEAFKQHLGVQYAIATS
SCTGALHMGMHALGIGPGDEVILADTNWIATAAPIIHLGAIPVFVDILPDSWCLDPEQVQ
AAITPKTKAIIAVHLYGNLCEMDALLAIGERYSIPVIEDAAEAIGSVYHGKRAGSMGRFG
AFSFHGTKTITTGEGGMFVTNDPELYEHVLTLSNHGRARGQTKQFWPDMVGFKYKMSNIQ
AAIGYAQMQRIEILIHMKRKIFSMYQNYLKDLPVQMNHEPRGCTNGYWMPTFVVNEKVSF
NRNKLISKLKSNGVDARVFFWPLSSTSTGGRKALDKPYLSESIHLHALNLPSHYQLSNEE
SMFICTLIKNTSFSG